Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chongqing Medicine》 2007-09
Add to Favorite Get Latest Update

Expression IL-2 and TNF-α after implanted to subcutanenous tissue with allogeneic tissue engineered bone

LIANG Wen-jie,XIE Zhao,WU Xue-hui,et al.(Department of Orthpaedics,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To explore the feasibility of allogeneic mesenchymal stem cells(MSCs) as seed cells to construct tissue engineered bone by detecting the expressions of IL-2 and TNF-α to Chinese minipig strain of Xiangzhu after implanting these tissue engineered bones.Methods Engineered bones were constructed with osteoblasts which derived from allogeneic MSCs and DBM in vitro,and then were implanted to subcutanenous tissue in left back as experimental group,DBM only were implanted to right as control group.Every 3 minipig strain of Xiangzhu were sacrificed in 1,2,4,8,12 week postoperatively and the expressions of IL-2 and TNF-α in blood and graft samples were detected quantitatively by enzyme-linked immuneosorbent assay(ELISA).Results There was no significant difference in the contents of IL-2 and TNF-α between two groups.The contents of IL-2 and TNF-α increased from 2 week and maintained high level from 2-4 weeks,but decreased after 8 weeks.Conclusion Tissue engineered bones constructed with allogeneic MSCs and DBM show low immunogenicity.Allogeneic MSCs may be used as seed cells to construct tissue engineered bone.
【Fund】: 国家高技术研究发展计划资助重大攻关课题(863计划)(2006AA02A122)
【CateGory Index】: R318
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 CHAI Gang, ZHANG Yan , LIU Wei, CUI Lei, CAO Yi-lin.Department of Plastic and Reconstructive Surgery, Ninth People′s Hospital, Shanghai Second Medical University, Shanghai Tissue Engineering Center, Shanghai 200011, China;Clinical application of tissue engineered bone repair of human craniomaxillofacial bone defects[J];National Medical Journal of China;2003-19
2 YANG Zhiming,LI Yanlin,XIE Huiqi,QIN Tingwu,HUANG Fuguo.Department of Orthopedic Surgery,the First University Hospital,West China University of Medical Sciences,Chengdu 610041,China\;;Experimental studies on histocompatibility of three bio-derived bones[J];Chinese Journal of Plastic Surgery and Burns;2002-01
3 SHEN Bing, XIE Fu lin, XIE Qing fang, et al. The First Orthopedic Hospital of Chengdu. Chengdu Sichuan, P. R. China 610015;COMPARATIVE STUDY ON GRAFT OF AUTOGENEIC ILIAC BONE AND TISSUE ENGINEERED BONE[J];Chinese Journal of Reparative and Reconstructive Surgery;2002-06
4 YANG Fu chun,YANG Zhi ming,LI Xiu qun,et al. Department of Orthopaedic Surgery, West China Hospital, Sichuan University. Chengdu Sichuan, P. R. China, 610041;REPAIR OF SEGMENTAL BONE DEFECTS IN RHESUS MONKEYS' RADIUS WITH ALLOGENEIC TISSUE ENGINEERED BONES[J];Chinese Journal of Reparative and Reconstructive Surgery;2003-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GAO Chun-yang,LI Qi-hong,JIAN Yue-kui,et al.(Department of Orthopedics,Southwest Hospital,the Third Military Medical University,Chongqing 400038,China);Experimental study on properties of xenogeneic deproteinised cancellous bone as scaffold of bone tissue engineering[J];Chongqing Medical Journal;2005-12
2 FENG Jiang-hua,XIE Zhao,WU Xue-hui,et al.(Department of Orthopedics,Orthopedic Center of PLA,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Osteogenic effect of heterogenic rat MSCs: a preliminary study[J];Chongqing Medicine;2007-09
3 YANG Zhi ming (Division of Stem Cell and Tissue Engineering,Key Laboratory of Biotherapy of Human Diseases,Ministry of Education.P.R.China, West China Hospital,Sichuan University,Chengdu 610041,China);Application of tissue engineering in trauma repair[J];Journal of Traumatic Surgery;2004-02
4 ZHANG Chen,WANG Zhi-jun,GAO Jing-heng(Xinhua Hospital Affiliated of Dalian University,Dalian 116021,China);Current Status of the Study of the Acellular Tissue Matrix[J];Journal of Dalian University;2007-03
5 ;A dvances in research and exploitation of bone tissue engineering[J];Acta Academiae Medicinae Militaris Tertiae;2005-16
6 LUO Fei, XU Jian-zhong, WANG Xu-quan, XIE Zhao, DAI Fei, HE Qing-yi, YIN Zhi-hua (Department of Orthopedics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effect of autologous serum on the proliferation of hMSCs adapted with calf serum[J];Acta Academiae Medicinae Militaris Tertiae;2005-16
7 CAO Kai~1,HUANG Wei~1,AN Hong~1,JIANG Dian-ming~1,HUANG Lu~2 (~1Department of Orthopaedics,The First Affiliated Hospital,~2Department of Renal Diseases,The Children's Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China);Deproteinized bone with VEGF gene transfer for avascular necrosis of the femoral head[J];Acta Academiae Medicinae Militaris Tertiae;2006-03
8 LIU Lei, LI Qi-hong, TANG Kang-lai, YANG Liu, JIAN Yue-kui (Department of Orthopaedics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);On biocompatibility of heterogeneous deproteinized bone as scaffold material[J];Acta Academiae Medicinae Militaris Tertiae;2006-22
9 LIU Lei,LI Qi-hong,TANG Kang-lai,YANG Liu,JIAN Yue-kui (Department of Joint Surgery,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Establishment of scaffold materials of deproteinized heterogeneous bone and their physicochemical properties[J];Acta Academiae Medicinae Militaris Tertiae;2007-01
10 CHEN Bin1, PEI Guo-xian1, WANG Ke2, TANG Guang-hui1 1Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2Department of Orthopedics, 153 Hospital of PLA, Zhengzhou 450042, China;Long-term observation of large weight-bearing bone defect in goats repaired with tissue engineering technique[J];Journal of Southern Medical University;2006-06
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Ding Zhen- qi,Tan Fu-sheng, Wu Zu-yao, et al.Dept.of Orthopedic Surgery, 1st Affiliated Hospital,Chongqing University of Medical Sciences, chongqin 630042;A Comparison of Healing in Four Graft Materials in Rabbit Skull Defects[J];CHINESE JOURNAL OF TRAUMATOLOGY;1994-06
2 YANG Zhiming,LI Yanlin,XIE Huiqi,QIN Tingwu,HUANG Fuguo.Department of Orthopedic Surgery,the First University Hospital,West China University of Medical Sciences,Chengdu 610041,China\;;Experimental studies on histocompatibility of three bio-derived bones[J];Chinese Journal of Plastic Surgery and Burns;2002-01
3 YANG Zhi-ming, ZHAO Yong-fan, XIE Hui-qi, et al. Department of Orthopedic Surgery, the First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P. R. China 610041. E-mail:orthop@public.cd.sc.cn;HUGE THORACIC WALL DEFECT REPAIRED BY TISSUE ENGINEERED BONE TRANSPLANTATION[J];Chinese Journal of Reparative and Reconstructive Surgery;2000-06
4 YANG Zhi ming, HUANG Fu guo, QIN Ting wu, et al. Key Laboratory of Transplant Engineering and Immunology of Ministry of Health:Institute of Reparative and Reconstructive Surgeryab of Tissue ngineering. West China Hospital, Sichuan University. Chengdu S;BIO-DERIVED BONE TRANSPLANTATION WITH TISUE ENGINEERING TECHNIQUE: PRELIMINARY CLINICAL TRIAL[J];Chinese Journal of Reparative and Reconstructive Surgery;2002-05
5 Li Yanlin, Yang Zhiming. Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences. Chengdu Sichuan, P.R. China 610041. E mail: orthop @ public.cd.sc.cn;CERAMIC LIKE XENOGENEIC BONE COMBINING WITH BONE MARROW[J];CHINESE JOURNAL OF REPARATIVE AND RECONSTRUCTIVE SURGERY;1999-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved