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《Chongqing Medicine》 2007-19
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Establishment of a lung cancer cell line marked by green fluorescent protein

CHEN Fang-lin CHEN Zheng-tang (Cancer Institute of PLA,Xinqiao Hospital,Third Military Medical University,Chong qing 400037,China)  
Objective In order to observe the infiltration and metastasis of the tumor,establish a high-metastatic lung cancer cell line expressing enhanced green fluorescent protein(EGFP).Methods 95D cell line was infected by the retrovirus which mediating expression of an enhanced green fluorescent protein gene and selected by G418.The expression of EGFP of the infected cells was observed by fluorescence microscopy and detected by flow cytometry.Multiple biological behaviors,such as the growth curve and cell adherent rate were compared between the infected and uninfected cells.Results 95D cell line could be infected efficiently by retrovirus.The selected cells could express the EGFP stably,efficiently and persistently.There was no significant difference be- tween infected cells and uninfected cells in their biological behaviors.Conclusion The establishment of 95D/GFP-1 can offer a use- ful cell line to investigate the mechanisms of tumor's infiltration and metastasis.
【CateGory Index】: R734.2
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【References】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Xin-sheng1,LIU Xiao-yu1,REN Shuang-yi2(1.Seven-Year Program of Clinical Medicine,Dalian Medical University,Dalian 116044,China;2.Department of General Surgery,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China);Discovery and application of green fluorescent protein[J];Journal of Dalian Medical University;2009-04
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【Co-references】
Chinese Journal Full-text Database 5 Hits
1 WANG Xiao-li1,SHAO Wei-xing2,SHAN Hu1(1.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao,Shandong,266109,China;2.China Animal Health and Epidemiology Center,Qingdao,Shandong,266071,China);Advance in Green Fluorescent Protein[J];Progress in Veterinary Medicine;2008-01
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5 LI Ren-fei*,SHEN Bao-zhong,WANG Zhi-fei,HE Neng-shu.*Department of Radiology,The Third Affiliated Hospital of Harbin Medical University,Harbin 150040,China Corresponding author:SHEN Bao-zhong,Email:cjr.shenbaozhong@vip.163.com;Molecular imaging research on the effect of endostatin on mice GFP-expressing Lewis lung carcinoma[J];Chinese Journal of Radiology;2004-04
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