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Establishment of a lung cancer cell line marked by green fluorescent protein

CHEN Fang-lin CHEN Zheng-tang (Cancer Institute of PLA,Xinqiao Hospital,Third Military Medical University,Chong qing 400037,China)  
Objective In order to observe the infiltration and metastasis of the tumor,establish a high-metastatic lung cancer cell line expressing enhanced green fluorescent protein(EGFP).Methods 95D cell line was infected by the retrovirus which mediating expression of an enhanced green fluorescent protein gene and selected by G418.The expression of EGFP of the infected cells was observed by fluorescence microscopy and detected by flow cytometry.Multiple biological behaviors,such as the growth curve and cell adherent rate were compared between the infected and uninfected cells.Results 95D cell line could be infected efficiently by retrovirus.The selected cells could express the EGFP stably,efficiently and persistently.There was no significant difference be- tween infected cells and uninfected cells in their biological behaviors.Conclusion The establishment of 95D/GFP-1 can offer a use- ful cell line to investigate the mechanisms of tumor's infiltration and metastasis.
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