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《Chongqing Medicine》 2007-20
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Effect of TGF-β_1 on human kidney tubular epithelial cells′ aptoptosis and cell proliferation dependent of the smad signaling pathway

REN Yun-zhuo,HAO Jun,SI Yong-hong,et al.(Department of Pathology,Hebei Medical University,Shijiazhuang 050017,China)  
Objective To examine the effect of TGF-β1 stimulation on smads signaling pathway of human kidney tubular epithelial cells.Methods HKC was stimulated by the recombinant human TGF-β1.The levels of p-smad2/3 and smad2/3 protein were examined by Western blot.At the same time.The level of smad2/3 mRNA was analyzed by reverst transcription polymerase chain reaction(RT-PCR).Flow cytometry was employed to investigate cells′ apoptosis and cycle.MTT assay was used to measure the proliferation of cells at 6,12,24 and 48 hours.Results The protein of p-smad2/3 and smad2/3in HKC obviously increased after TGF-β1 stimulation.TGF-β1 stimulation induced apoptosis of HKC.At the same time,The percent of G0/G1 phase of HKC cell cycle increased apparently.Meanwhile,the proliferation of HKC was markedly inhibited from 6 hour.Conclusion(1)The smad signaling pathway of HKC can be specifically activated by TGF-β1.(2)TGF-β1/Smad signaling patheway may partly cause HKC′s apoptosis and inhibit its proliferation.
【Fund】: 河北省自然科学基金资助项目(C2006000825)
【CateGory Index】: R692.6
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【References】
Chinese Journal Full-text Database 5 Hits
1 JIANG Qing,WAQNG Ping-xian(Department of Urology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Investigation for influence of TGF-β_1 on long-term renal allograft function[J];Chongqing Medicine;2008-14
2 Wang Mingyong1,Yuan Bo2,Xia Jiyi1,Chen Feng1,Chen Zhuang1,Liu Jian3(1.Medical Experimental Center,Affiliated Hospital,Luzhou Medical College,Luzhou,Sichuan 646000,China;2.Yibin First Pople′s Hospital,Yibin,Sichuan 644000 China;3.Departmen of Nephrology,Affiliated Hospital,Luzhou Medical College,Luzhou,Sichuan 646000,China);Role of ERK1/2 signaling pathway on angiotensin-(1-7) inhibit rat′s tubular epithelial myofibroblast transdifferentiation induced by angiotensinⅡ[J];Chongqing Medicine;2011-09
3 REN Yun-zhuo,WANG Chen,SHI Yong-hong,DUAN Hui-jun(Dept of Pathology,Hebei Medical University,Shijiazhuang 050017,China);Effect of valsartan on expression of TGF-β/Smads signaling pathway in human kidney proximal tubular epithelial cell[J];Chinese Pharmacological Bulletin;2008-07
4 GUO Hao,YANG Da-kuan,TIAN Qing,et al.Department of Cardiology,The First People s Hospital of Kunming City and Affiliated Calmette Hospital of Kunming Medical College,Kunming 650011,China;Relationship between Symmetric Ambulatory Arterial Stiffness Index and Urine n-acetyl-β-d-Glucosaminidase in Patients of Essential Hypertension[J];Medicine & Philosophy(Clinical Decision Making Forum Edition);2010-12
5 WEI Zhi-qiang,TU Wei-ping,FANG Xiang-dong(Department of Nephrology,the Second Affiliated Hospital of Medical College of Nanchang University,Nanchang,Jiangxi 330006,P.R.China);Research progress on inducer of promoted Epithelial to Mesenchymal Transdifferentiation[J];China Journal of Modern Medicine;2012-02
【Citations】
Chinese Journal Full-text Database 2 Hits
1 LIANG Zhi qing,XU Xin bao,HE Zhen ping (Hepatobiliary Surgery Center,Southwest Hospital, the Third Military Medical University,Chongqing 400038);The expression and the role of TGF-β receptors and signal transduction molecule Smad4 in rat fibrotic liver[J];Chongqing Medical Journal;2002-07
2 HUANG Hongling, ZHANG Guoqiang,YE Rengao, et al. Department of Nephrology, Key Clinical Kidney Research Institnte of Minitry of Health, First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510089, China;The role of cell proliferation and apoptosis in renal tubulointerstitial fibrosis caused by unilateral ureteral obstruction[J];Chinese Journal of Nephrology;2000-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Zhi, YAN Guo-fu, HU Mei-ling,et al. (Department of Dermatology,Xinqiao Hospital,the Third Military Medical University,Chongqing 400037,China);Mouse model of sclerotic skin induced by local injections of bleomycin[J];Chongqing Medical Journal;2004-12
2 ZHANG Jian-Jiang,YI Zhu-Wen,DANG Xi-Qiang, HE Xiao-Jie,WU Xiao-Chuan.Laboratory of Pediatric Nephrology,Second Xiangya Hospital,Cenrtal South University,Changsha 410011,China;Mobilization effects of SCF along with G-CSF on bone marrow stem cells and endothelial progenitor cells in rats with unilateral ureteral obstruction[J];Chinese Journal of Contemporary Pediatrics;2007-02
3 HUA Yun-peng1, LI Shao-qiang1, LAI Jia-ming1, LIANG Li-jian1, PENG Bao-gang1, LIANG Hui-zhen2, HUANG Jie-fu1 1Department of Hepatobiliary Surgery, 2Department of Pathology, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China;Changes in TGF-β1/Smads signaling pathway in rats with chemical hepatocarcinogenesis[J];Journal of Southern Medical University;2008-10
4 PENG Xiao-lan,DONG Xiao-kai,LIAO Fang-fang,GAO Lei,ZHANG Zhong-wen,WU Guo-juan(Animal Science and Technology College,Beijing University of Agriculture,Beijing 102206,China);Investigate the Effect of Aristolochic Acid Ⅰ in Inducing Apoptosis of the RTECs[J];China Animal Husbandry & Veterinary Medicine;2011-11
5 Wang Mingjun.(Department of Nephrology, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021 China);EXPERTIMENTAL STUDY FO LEFLUNOMIDE IN PREVENTING RENAL INTERSTITIAL FIBROSIS IN RATS[J];Journal of Guangxi Medical University;2007-02
6 SUN Jian~ 1 ,TAO Li-jian~ 1,* , JIN Ou~ 2 , NING Wang-bin~ 1 ,DAMU Tang~ 3 (1.Department of Nephrology, Xiangya Hospital, Central South University; 2.Department of Pathology, Basic Medical College, Central South University, Changsha 410008;3.Department of Medicine, McMaster University, Hamilton, Ontario L8N 4A6, Canada);Expression of p27 in rat kidney with unilateral ureteral obstruction and the therapeutic effect of enalapril[J];Journal of Central South University(Medical Sciences);2006-05
7 HAN Lin1,QIN Jian-guo2,CHEN Zhi-qiang3,XU Qing-you3,LI Peng-tao4(1 School of Fundamental Medicine,Beijing University of Chinese Medicine,Beijing 100029;2 Dongfang Hospital,Beijing University of Chinese Medicine;3 Institute of Integration of Chinese and Western Medicine,Hebei Medical University;4 Dongzhimen Hospital,Beijing University of Chinese Medicine);Influence of Biejiajian Pills on cell proliferation in rats with renal interstitial fibrosis[J];Journal of Beijing University of Traditional Chinese Medicine;2009-07
8 YUE Bing-de,GUAN Ying-jun,SUN Xiu-ning,GAO Hai-ling (Department of Histology and Embryology,Weifang Medical College,Weifang 261041,China);A Study on the Relationship between the Cell Proliferation and Apoptosis in the Kidey of Diabetic Mice[J];Acta Academiae Medicinae Weifang;2006-03
9 Wang Zhaolin, Ma Hua (The Second Hospital of Shanxi Medical University, Taiyuan Shanxi 030001);Effect of Qihong Decoction on bcl-2 Expression in Renal Cortex of Model Rats with Tubulointerstitial Fibrosis[J];Journal of Shanxi College of Traditional Chinese Medicine;2006-04
10 MA Zu-xiang 1, ZHAO Wei-ling 1, HE Xiao-jie 2,YI Zhu-wen 2 (1.Department of Nephrology, Shenzhen Children′s Hospital, Shenzhen 518026,China;2.Department of Pediatrics,the Second Xiangya Hospital, Zhongnan University,Changsha 410011,China);Expression of proliferating cell nuclear antigen in renal tissues of children with primary nephrotic syndrome[J];Journal of Applied Clinical Pediatrics;2004-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 SHAN Bao_en 1 ,LIANG Wen_jie 1 ,LIU Dong_qing 2 ,REN Feng_zhi 3 (1.Research Center,The Fourth Hospital of Hebei Medical University,Shijizhuang,2.The First Hospital of Handan; 3.Northchina Phamacal coperation,050011,China);Effect on Apoptosis of BGC823 Cells Induced by Rhizoma Menipermi Extracts-PE2[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-04
2 SHAN Bao-en1,LIU Dong-qing2,LIANG Wen-jie1,ZHANG Jing1,LI Qiao-xia1(1.Research Center of the Fourth Hospital of Hebei Medical University,Shijiazhuang,050011,China;2.The First Hospital of Handan);The Anti-tumor Effect and Immunoregulatory Activity of PE2 From Rhizoma Menipermi Extracts in Vivo[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-05
3 SUN Suozhu1) CHENG Bo1) WU Xizhao1)ZHOU Yanmin1) ZOU Wanzhong 2)(1)Department of Pathology,General Hospital of the Second Artillery,PLA,100088,Beijing,China;2)Department of Pathology,Peking University Health Science Center,100083,Beijing,China);RENAL EPITHELIAL CELL APOPTOSIS,PROLIFERATION,TRANSDIFFERENTATION AND TGFβ1 EXPRESSION IN RAT URIC ACID NEPHROPATHY[J];Journal of Beijing Normal University(Natural Science);2008-03
4 LI Ling, ZOU Wanzhong △, ZHANG Bo,WANG Shenglan, GUO Hua (Department of Pathology,Peking University School of Basic Medical Sciences, Beijing 100083,China);The phenotypic transformation of renal tubular epithelial cells affected by pathological stimuli[J];Journal of Beijing Medical University;2003-01
5 LI Dan,DING Jie (Department of Pediatrics,Peking University First Hospital,Beijing 100034,China);Molecular mechanism of proteinuria caused progression of chronic renal disease[J];Journal of Peking University(Health Sciences);2010-05
6 WANG Ping-xian,HUANG Chi-bing,FAN Ming-qi,et al.(Department of Urology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Delaying progression of chronic allograft nephropathy by conversion from cyclosporine to tacrolimus[J];Chongqing Medicine;2006-15
7 Hong GAO, Chang-Lin WANG, Wei-Song CAI, Bei-Bei ZHU. Major Laboratory of Health Ministry in Congenital Malformations, Second Affiliated Hospital, China Medical University, Shenyang 110004, China;Expression of Survivin and Caspase-3 mRNA in Wilms tumor[J];The Chinese Journal of Contemporary Pediatrics;2004-05
8 QIAN Ming,GAN Wei-Hua,CHEN Rong-Hua,PAN Xiao-Qin,FEI Li.Institute of Pediatrics,Nanjing Medical University,Nanjing 210029,China;Transdifferentiation and collagen-synthesis effects of exogenous connective tissue growth factor on renal tubular epithelial cells in vitro[J];Chinese Journal of Contemporary Pediatrics;2006-02
9 FU Shu-xia,WANG Xi-qian,HAO Yu-jie,YANG Lin.Department of Nephrology,The Second Hospital of Hebei Medicine University,Shijiazhuang 050000,China;Protective effects and mechanism of Pentoxifylline on rats with renal interstitial fibrosis following unilateral ureteral obstruction[J];Journal of Chinese Physician;2006-05
10 WU Hong Mei, LING Chang Quan, ZHU De Zeng (Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433,China) [;Examining the pulses manifestation of patients with primary hepatocarcinoma[J];Academic Journal of Second Military Medical University;2001-07
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 LI Feng1,XIAO Ya2,WANG Ping-xian2(1.Department of Urology,Sanxia Center Hospital,Chongqing 404000,China;2.Department of Urology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Slowing progression of chronic allograft nephropathy by conversion from cyclosporine to tacrolimus[J];Chongqing Medicine;2009-17
2 JIANG Qing1,WANG Ping-xian2,ZHANG Yin-fu2△(1.Department of Urology,The Second Affiliated Hospital,Chongqing Medical University,Chongqing 400030,China;2.Department of Urology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Effect of losartan on post-renal transplant erythrocytosis of 136 cases[J];Chongqing Medicine;2010-12
3 ZHANG Suwan,LI Sumei(Department of Endocrinology,Anhui Provincial Hospital Affiliated to Anhui Medical University,Hefei 230001,China);Role of p38 mitogen-activated protein kinase in inflammatory cytokines-induced diabetic nephropathy[J];International Journal of Pathology and Clinical Medicine;2011-01
4 Yang Lei1, Guo Juan1, Zhu Ming-hui2, Miao Shu-zhai1, Qu Qing-shan1, Guo Kai2 1Department of Organ Transplantation, 2Department of Clinical Laboratory, People's Hospital of Zhengzhou, Zhengzhou 450003, Henan Province, China;Thirty-five kidney transplant recipients after conversion to tacrolimus from ciclosporin A:A one-year follow-up[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2010-44
5 WANG Li-hui1,DUAN Hui-jun2,SHI Yong-hong2,LIU Qing-juan2,LIU Shu-xia2(1.Dept of Nephrology,Bethune International Peace Hospital,Shijiazhuang 050082,China;2.Dept of Pathology,Hebei Medical University,Shijiazhuang 050017,China);Effects of valsartan on expression of p38MAPK signal transduction pathway in glomerular mesangial cells under high concentration of glucose[J];Chinese Pharmacological Bulletin;2009-04
6 LI Hang1,3,CAO Yan-ping2,ZHANG Lian-shan3,CHEN Wei1, WANG Hui-juan1,ZHANG Jin-ping1,DUAN Hui-jun3(1.Dept of Histology and Embryology,Hebei Medical University,Shijiazhuang 050017,China; 2.Dept of Pathology,Traditional Medicine Hospital of Hebei Province,Shijiazhuang 050011,China; 3.Dept of Pathology,Hebei Medical University,Shijiazhuang 050017,China);tBHQ attenuates high glucose induced oxidative stress injury in mouse mesangial cells[J];Chinese Pharmacological Bulletin;2011-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zheng Guoqiang, Ye Rengao , Cheng Yongxinng, et al. Institute of Kidney, Sun Yat-sen University of Medical Sciences, Guangzhou 510089;Comparison of growth and apoptosis of fibroblasts derived from kidneys with and without interstitial fibnwis[J];Chinese Journal of Nephrology;1997-02
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