Ultrastructural observation of adriamycin induced focal segmental glomerularsclerosis in rats
ZHAO Hong-wen,YU Rong-jie,PENG Kan-fu,et al.(Department of Kidney,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)
Objective To observe the ultrastructural changes of adriamycin induced focal segmental glomerularsclerosis in rats.Methods Female SD rats were divided into normal control group and adriamycin renal disease (ADR) group randomly.ADR group were carried out unilateral nephrectomy and injected with 5mg/kg and 3mg/kg of adriamycin via tail vein at the 1st and 2nd week after nephrectomy,while the control group rats were carried out sham operation and 0.9% Sodium Chloride solution injection of equal volume.The urine protein and Serum creatinine (Scr) were measured 0w (before operation),4w,8w,and 16w after nephrectomy,while the renal histological and ultrastructural changes were evaluated.Results The proteinuria and Scr were more severe in ADR group than that in control group.In ADR group,mesangial proliferation and the tubular-interstitial fibrosis were more remarkable than that in control group.The results of electron microscope showed that the podocytes leisions in ADR group were more severe than that of control group at the 16th week,and the main changes were focused on podocytes and tubular epithelial cells.Conclusion After the second injection,the changes of kidney are like those in minimal change glomerulonephritis of human being.The kidney have the changes of focal segmental glomeralosclerosis.
【CateGory Index】： R692