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Effect of hyperglycemia on the expression of glucose transporter 3 in rats following traumatic brain injury

WANG Liang,TANG Wen-yuan,YANG Gang,et al.(Department of Neurosurgery,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China)  
Objective To explore the mechanism about neuroprotection of insulin therapy in rats at the acute stage of traumatic brain injury.Methods Adult male SD rats were randomly divided into 3 groups:normal control group,traumatic brain injury group and insulin therapy group,the blood glucose concentration was measured before and after injury.RT-PCR was applied to detect the expression of GLUT3 genes,The changes of GLUT-3 and NSE positive-staining cells were examined by immunohistochemistry.Results The blood glucose concentration only increased markedly in traumatic brain injury group.through the expression of GLUT-3 and NSE positive-Staining cells decreased in the injured cortical both in traumatic brain injury group and insulin therapy group,there was much more expression of GLUT-3 but less NSE positive-Staining cells loss was assessed at 12 h,24 h,48 h,72 h after injury in the insulin therapy group.There were no changes were found in the uninjured side of each group.Conclusion Insulin therapy at the acute stage of traumatic brain injury shows certainly neuroprotection by decreasing the neurons loss,the mechanism may be assossiated with down regulation of GLUT-3 expression which depressed by hyperglycemia.
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