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《Chongqing Medicine》 2010-15
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Effect of TSA on the expression of FHIT in the human hepatoma cell line HepG2

WANG Li,LIU Xiao-yan,GONG Shu,et al.(Laboratory of Medical Molecular Biology,Luzhou Medical College,Luzhou,Sichuan 646000,China)  
Objective To investigate the effect of TSA on the expression of fragile histidine triad(FHIT) in the human hepatoma cell line HepG2.Methods After the human hepatocarcinoma cells and the normal liver cells had been treated with various concentrations of TSA in different duration in vitro,cell morphologies were observed by the inverted light microscope,and cell viability were analyzed by MTT assay.The expression of FHIT was analyzed by RT-PCR and Western blot. Results TSA inhibited the proliferation of HepG2 cells in dose-dependent and time-dependent manners,but the normal liver cell line LO2 was not sensitive to the TSA.The expression of FHIT mRNA and FHIT protein were increased after treatment of TSA(P0.05).Conclusion The alternation of FHIT gene correlated with abnormal histone deacetylation and TSA could inhibit the proliferation of the hepatocarcinoma cell line HepG2,which were associated with inhibition of enzymatic activity of HDACS,regulation of FHIT gene transcription and FHIT protein expression.
【Fund】: 四川省卫生厅科研项目(060065)
【CateGory Index】: R735.7
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 HE Song,ZUO Guo-qing,ZHANG Yan,et al.(1.Department of Gastroenterology,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;2.Department of Pathophysiology,Chongqing Medical University,Chongqing 400016,China);Effects of matrine on proliferation and telomerase activity in human hepatocellular cell line HepG2[J];Chongqing Medicine;2008-03
2 XIE Jing,et al (Department of Gastroenterology,the Second Affiliated Hospital,Chongqing Medical University);Effects of trichostatin A(TSA) on the proliferation and apoptosis of hepatoma cell lines and the the replication of hepatitis B virus(HBV)[J];Journal of Chongqing Medical University;2008-09
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 HUANG Jin-fei JIA Jian-wen LUO Jian-jun WENG Qun-fang ZHONG Guo-hua Laboratory of Insect Toxicology,South China Agricultural University,Guangzhou 510642,China;Modes of Action of Six Different Botanical Alkaloids against Spodoptera litura Cultured Cell Line SL-1[J];Journal of Huazhong Agricultural University;2010-01
2 QIN Kunming,FANG Qianbo,CAI Hao,CAI Baochang (Jiangsu Key Laboratory of Chinese Medicine Processing,Nanjing University of Chinese Medicine,Nanjing 210029,China);Summary of Research on Anticancer Mechanism of Constituent Matrine[J];Journal of Shenzhen Polytechnic;2009-03
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【Secondary Citations】
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1 SHEN Rong,TAO Yi-sheng (Department of Pathology,Bengbu Medical College,Bengbu 233030,China);The effects of sodium butyrate on proliferation and apoptosis of esophageal carcinoma Eca-109 cells[J];Journal of Bengbu Medical College;2006-04
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3 Tang Weixue,et alDepartment of Pathophys iology;DETERMINATION OF ANTICANCER DRUG SENSITIVITY TEST BY THE MTT COLO RIMETRIC ASSAY FOR HUMAN SOLID TUMORS[J];Journal of Chongqing Medical University;1992-02
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