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《Chongqing Medicine》 2010-15
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Effect of TSA on the expression of FHIT in the human hepatoma cell line HepG2

WANG Li,LIU Xiao-yan,GONG Shu,et al.(Laboratory of Medical Molecular Biology,Luzhou Medical College,Luzhou,Sichuan 646000,China)  
Objective To investigate the effect of TSA on the expression of fragile histidine triad(FHIT) in the human hepatoma cell line HepG2.Methods After the human hepatocarcinoma cells and the normal liver cells had been treated with various concentrations of TSA in different duration in vitro,cell morphologies were observed by the inverted light microscope,and cell viability were analyzed by MTT assay.The expression of FHIT was analyzed by RT-PCR and Western blot. Results TSA inhibited the proliferation of HepG2 cells in dose-dependent and time-dependent manners,but the normal liver cell line LO2 was not sensitive to the TSA.The expression of FHIT mRNA and FHIT protein were increased after treatment of TSA(P0.05).Conclusion The alternation of FHIT gene correlated with abnormal histone deacetylation and TSA could inhibit the proliferation of the hepatocarcinoma cell line HepG2,which were associated with inhibition of enzymatic activity of HDACS,regulation of FHIT gene transcription and FHIT protein expression.
【Fund】: 四川省卫生厅科研项目(060065)
【CateGory Index】: R735.7
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