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Correlation of ERK signal transduction pathway with MMP-9 expression in lung adenocarcinoma

LI Lian1,LI Ming2,ZHOU Xiang-dong3(1.Department of Respiratory Medicine,Jiangjin District People′s Hospital,Jiangjin,Chongqing 402260,China;2.Department of Pathology,Jiangjin District People′s Hospital,Jiangjin,Chongqing 402260,China;3.Department of Respiratory Medicine,Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China)  
Objective To investigate the correlation of extracelluar signal-regulated kinase(ERK) with matrix metalloproteinase-9(MMP-9)and its significance in lung adenocarcinoma.Methods SP immunohistochemistry was used to test the expression of pERK and MMP-9 in 67 specimens of lung adenocarcinoma.Western blot was used to detect the protein levels of MMP-9 in lung adenocarcinoma SPC-A-1 cells after blocking ERK signal transduction pathway by PD98059.The correlation of pERK and MMP-9 expression was also analyzed.Results The high expression rate of pERK proteins was higher in lung adenocarcinoma tissues than in the normal tissues(65.6%vs 14.3%).The high expression rate of MMP-9 protein was higher in lung adenocarcinoma tissues than in the normal tissues(73.1%vs 21.4%).The high expression rate of pERK was positively correlated to the high expression rate of MMP-9(r=0.57,P0.05).The high expression rate of pERK was correlated to lymph node metastasis and TNM stage(P0.05),but not to patients,age and tumor size(P0.05).The high expression rate of MMP-9 was correlated to lymph node metastasis and TNM stage(P0.05),but not to patient′s age and tumor size(P0.05).PD98059 concentration-dependently inhibited ERK pathway and reduced MMP-9 protein expression.Conclusion ERK signal transduction pathway might promote lung adenocarcinoma progression by up-regulating MMP-9 expression,and might be an important route in invasion and metastasis of lung adenocarcinoma.
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