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《Chongqing Medicine》 2012-19
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Preliminary study of relationship between radiation-induced mitochondrial DNA 4 977 bp deletion and radiosensitivity of human tumor cell lines

Rong Qinglin1,Liu Li2,Wang Qin3,Liu Qiang3,Chen Yanfang1,Jiang Wenhua1,Li Xiaodong1(The Second Hospital of Tianjin Medical University:1.Department of Radiation Oncology;2.Tianjin Institute of Urology,Tianjin 300211,China; 3.Key Laboratory of Molecular Nuclear Medicine,Institute of Radiation Medicine,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300192,China)  
Objective To explore the potential relationship of the radiation-induced mitochondrial DNA 4 977 base pairs(mtDNA 4 977 bp) deletion and the radiosensitivity of tumor cell lines.Methods The surviving fraction(SF) and the ratio of mtDNA 4 977 bp deletion were determined by clone forming assay and nested polymerase chain reaction(PCR) technique respectively after tumor cells of Hela,HepG2,EC-9706 and PC-3 were irradiated by various doses of X-ray radiation from 0 Gy to 8 Gy.Results The descending order of the four tumor cell lines′ radiosensitivities were the Hela,HepG2,EC9706 and PC-3 cells.The radiation-induced mtDNA 4 977 bp deletion were detected after 1 Gy irradiated in Hela cells and 2 Gy irradiated in HepG2,EC-9706 and PC-3 cells.The deletion rate of the four tumor cell lines had no significant difference at 1 Gy(P0.05);but the deletion rate of Hela cells was significantly higher than that of the other tumor cell lines at 2 Gy(P0.01);at 4 Gy and 8 Gy,the deletion rate of Hela cells was significantly higher than that of the other tumor cell lines(P0.05),and the deletion rates of HepG2 and EC-9706 cells were higher significantly than that of PC-3 cells(P0.01).There were negative corralations between the SF and the deletion rates in Hela,HepG2 and EC-9706 cells(r=-0.951,P0.05;r=-0.976,P0.01;r=-0.986,P0.01).Conclusion The ratio of mtDNA 4 977 bp deletion may be an biological marker to predict the radiosensitivity of tumor cells.
【Fund】: 天津市自然科学基金资助项目(07JCYBJC09200)
【CateGory Index】: R730.5
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