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《Chongqing Medicine》 2014-25
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Establishment of platelet antigen panel cells and application in platelet alloantibody detection

Zhou Yan;Zhong Zhoulin;Liu Jinlian;Li Lilan;Shen Weidong;Wu Guoguang;Nanning Blood Center/Nanning Institute of Transfusion Medicine;  
Objective To establish the platelet antigen panel cells and to apply them in the detection and identification of platelet alloantibody.Methods Human platelet antigen(HPA)1-16 genotyping from 1 500un-related blood donors in Nanning area were performed by the polymerase chain reaction-sequence specific primers(PCR-SSP)technique,platelet antigen cells with O blood type were chosen to establish the panel cells of platelet antigen.The phenotype of the panel cells were verified by the reference sera from the 14 th platelet immunology workshop of the International Society of Blood Transfusion(ISBT).And then these panel cells were used in clinic to detect the platelet alloantibody and the samples from the 14 th and 15 th platelet immunology workshop of ISBT.Results Six platelet cells with consistent phenotype and genotypes and covering the HPA 1-5and 15 systems were selected to establish the platelet panel cells and successfully applied them in the clinical and scientific sample detection and identification.Conclusion Platelet antigen panel cells are established successfully,which provides the experimental basis for the diagnosis and research of platelet allogenic abnormal immunity diseases.
【Fund】: 广西科技厅主席资金项目(08160-05);; 南宁市科技局项目(201003048C-2)
【CateGory Index】: R446.6
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