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《Chongqing Medicine》 2015-35
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The influence of RNAi on invasion and migration of gastric cancer cell line SGC-7901

Pei Bo;Cun Yingli;Yao Qian;Zhang Kailian;Qin Xiangnan;Dai Youguo;Zha Yong;Graduate School of Kunming Medical University;Department of Abdominal Surgery,the Third Affiliated Hospital of Kunming Medical University;Yunnan Tumor Institute,Tumor Hospital of Yunnan Province;  
Objective To investigate the effect of RNA interference(RNAi)on gastric cancer cell line SGC-7901 invasion and migration by inhibiting HER2 expression.Methods The RNAi segments targeting HER2 were designed and transfected into cell lines with high level of HER2(HER2-RNAi group),SGC-7901 cells transfected with nagetive control series(the nagetive control group)and vacant SGC-7901cells(the blank control group)were used as controls.The mRNA and protein expression of HER2 were detected by real-time PCR and Western blot.The invasive and migration ability of transfected tumor cells by Transwell assay and scratch assay.Results The expression of HER2 was significantly reduced after the transfection(P0.05).In invasion test,the number of cells crossing through chambers in HER2-RNAi group[(31.5±3.8)/view]was significantly lower than that in the control group[(103.6±4.5)/view](P0.05);in migration test,the number of cells crossing through chambem in HER2-RNAi group[(63.6±5.1)/view]was significantly lower than that in the control group[(193.5±6.2)/view](P0.05).Conclusion HER2 silencing by RNAi suppresses the capacities of invasion and migration of human gastric cancer SGC-7901 cells,suggesting that RNAi gene silencing technique might be a new method in the treatment of advance gastric cancer.
【Fund】: 云南省自然科学应用基础研究基金资助项目(2010CD179;2012FB065;2014FZ034)
【CateGory Index】: R735.2
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