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《Chongqing Medicine》 2017-15
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Influence of blocking gastrin receptor on the proliferation and apoptosis and expression of key proteins in related pathway of gastric cancer cell

Yuan Hang;Wang Chuang;Wang Qinrong;Zhao Yan;Li Yajie;Long Niya;Zhou Jianjiang;Key Laboratory of Molecular Biology of Guizhou Medical University/Key Laboratory of Endemic and Minority Diseases,Ministry of Education Guiyang;  
Objective To investigate the effects of blocking gastrin receptor on the proliferation,apoptosis and expression of key proteins in the related pathway in gastric cancer cell lines.Methods In the experimental group,the gastric cancer cell lines SGC-7901 and AGS cells were treated with 5mmol/L proglumide,a kind of a gastrin receptor antagonist.And the normal cultured gastric cancer cells SGC-7901 and AGS were used in control group.The growth of each group was detected by MTT assay;the cell growth curve was drawn by flow cytometry;the cell cycle of each group was detected by flow cytometry.Annexin V-FITC/PI double staining was used to detect the cell growth of apoptosis.The relative mRNA expression ofβ-catenin,nuclear factor-P65,mammalian target of rapamycin and glycogen synthase kinase 3beta in Wnt,NF-κB and PI3K-AKT-MTOR pathways were detected by RTqPCR.The expression ofβ-catenin protein was detected by Western blotting.Results After treatment with proglumide,the growth of the cells in the experimental group was lower than that in the control group;and the proportion of S phase cells in the cell cycle was also lower than that in the control group,but the proportion of cells in G_0/G_1 phase was higher than that in the control group(P0.05).The percentage of apoptotic cells was also increased after treatment with proglumide(P0.05).Furthermore,proglumide treatment significantly reduced the expression ofβ-catenin at both mRNA and protein levels(P0.05).Conclusion Blocking gastrin receptor can down-regulate the expression ofβ-catenin,inhibit the cell proliferation and promote the cell apoptosis in gastric cancer cells.
【Fund】: 国家自然科学基金资助项目(81260303 31560326);; 贵州省科技合作计划项目(黔科合LH字[2015]7360)
【CateGory Index】: R735.2
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Chinese Journal Full-text Database 5 Hits
1 Jia-Ding Mao;Pei Wu;Jian-Xiong Huang;Jian Wu;Guang Yang;Department of General Surgery,the First Affiliated Yijishan Hospital of Wannan Medical College;;Role of ERK-MAPK signaling pathway in pentagastrin-regulated growth of large intestinal carcinoma[J];World Journal of Gastroenterology;2014-35
2 LIU Jun1),ZHOU Jian-Jiang1),2) ,XIE Yuan1),ZHAO Yan1),ZENG Yi1)(1) Key Laboratory of Molecular Biology of Guiyang Medical College,Guiyang 550004,China; 2) Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China);Overexpression of Gastrin Promotes the Proliferation,Migration and Invasion of Gastric Cancer Cells[J];中国生物化学与分子生物学报;2013-04
3 LIU Jun1,ZHOU Jian-jiang1,2,ZHAO Yan1,XIE Yuan1(1Key Laboratory of Molecular Biology,2 Clinical Laboratory of Affiliated Hospital,Guiyang Medical College,Guiyang 550004,China);Gastrin promotes the migration and invasion of gastric cancer cells[J];中国病理生理杂志;2013-04
4 LIU Ying-Hui, SUO Jian, LI Shou-Bai, et al.Department of General Surgery, First Hospital of Jilin University, Changchun 130021, Jilin, China;Expression of β-catenin and NF-κB and their relation to gastric carcinoma[J];中国老年学杂志;2007-20
5 Jun Cao, Jie-Ping Yu, Hong-Gang Yu, Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China Chao-Hong Liu, State Key Laboratory of Virology, Wuhan institute of Virology, China Adcamy of Science, Wuhan 430060, Hubei Province, China Lan Zhou, Traditional Chinese Medicine Experiment Center, Hubei College of Traditional Chinese Medicine, Wuhan 4300061, Hubei Province, China;Effects of gastrin 17 on β-catenin/Tcf-4 pathway in Colo320WT colon cancer cells[J];World Journal of Gastroenterology;2006-46
Chinese Journal Full-text Database 8 Hits
1 Yuan Hang;Wang Chuang;Wang Qinrong;Zhao Yan;Li Yajie;Long Niya;Zhou Jianjiang;Key Laboratory of Molecular Biology of Guizhou Medical University/Key Laboratory of Endemic and Minority Diseases,Ministry of Education Guiyang;;Influence of blocking gastrin receptor on the proliferation and apoptosis and expression of key proteins in related pathway of gastric cancer cell[J];重庆医学;2017-15
2 CHEN Qing-fa;XU Yan;XUE Fang-xi;Department of Gastroenterology,Yishui Central Hospital of Linyi;;Effects of gastrin in the proliferation of human gastric cancer cell MKN45 and expression of osteopontin[J];临床和实验医学杂志;2017-06
3 WEI Hua;ZHANG Lei-Lei;LI Yan;SUO Zhi-Min;Department of Endoscopy,Huaihe Hospital,Henan University;;Value of PGⅠ,PGⅡ,G-17 and Hp IgG in screening of chronic atrophic gastritis and gastric cancer[J];中国免疫学杂志;2016-09
4 WU Ping;MAO Jiading;Department of Gastrointestinal Surgery,Yijishan Hospital,Wannan Medical College;;The role of JNK signal pathway in promoting colorectal cancer cells proliferation[J];胃肠病学和肝病学杂志;2016-02
5 ZHU Qi;XIONG Hua;ZHANG Rao;LU Hong;Aini·Abudureyimu;Department of Gastroenterology,Shanghai Jiao Tong University School of Medicine;Department of Gastroenterology, Kashgar Prefecture Second People's Hospital;;Analysis of the eradication G17 and PG content changes and clinical significance of Hp eradication in patients with Hp related chronic stomach disease[J];中国医学前沿杂志(电子版);2016-02
6 LIAO Jiang-tao;LI Juan;CHEN Yin-yun;Department of Gastroenterology,Older Hospital;;Expression and significance of GRP and GRPR in colorectal cancer[J];中国当代医药;2015-30
7 XIE Yuan;ZHAO Yan;LIU Zhengmei;GUAN Zhizhong;ZHOU Jianjiang;Key Laboratory of Molecular Biology,Guihzou Medical University;;Expression Analysis of Gastrin and Its Receptor in Gastric Cancer and Precancerous Lesions[J];贵阳医学院学报;2015-11
8 Chen Shaobin;Weng Hongrui;Wang Geng;Liu Ditian;Li Hua;Yang Jiesheng;Yang Weiping;Chen Yuping;Department of Surgery,Cancer Hospital of Shantou University Medical College;;Expression and clinical significance of gastrin receptor in adenocarcinoma of esophagogastric junction[J];中国医药;2015-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wanqing Chen 1 , Rongshou Zheng 1 , Siwei Zhang 1 , Ping Zhao 1 , Guanglin Li 2 , Lingyou Wu 2 , Jie He 1 1 National Office for Cancer Prevention and Control, National Cancer Center, Beijing 100021, China; 2 Bureau of Disease Prevention and Control, Ministry of Health, Beijing 100044, China;Report of incidence and mortality in China cancer registries, 2009[J];Chinese Journal of Cancer Research;2013-01
2 Eduardo Chueca,Angel Lanas,Elena Piazuelo,IIS Aragon,Aragon Institute of Health Sciences,50009 Zaragoza,Spain Angel Lanas,Elena Piazuelo,Networked Biomedical Research Center Hepatic and Digestive Diseases(CIBERehd),C/Corcega 180 bajos dcha,08036 Barcelona,Spain Angel Lanas,Elena Piazuelo,Department of Medicine,Psychiatry and Dermatology,Medical University of Zaragoza,C/Domingo Miral,50009 Zaragoza,Spain Angel Lanas,Service of Gastroenterology,Hospital Clinico Universitario,Avda.San Juan Bosco 15,50009 Zaragoza,Spain;Role of gastrin-peptides in Barrett's and colorectal carcinogenesis[J];World Journal of Gastroenterology;2012-45
3 SHI Xiao-yu1,TAO Si-feng2,CHEN Li1,PENG Shu-you1,HOANG-VU Cuong3 (1Department of General Surgery,2Department of Oncosurgery,The Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China.3Department of Surgery,Martin Luther University of Halle-Wittenberg,Halle 06099,Germany.);Effect of CD97 gene silencing by small interfering RNA on migration and invasion of gastric carcinoma cell lines[J];中国病理生理杂志;2011-07
4 ZHAO Yan1,XIE Yuan1,WANG Wenling2,WANG Su1,CHEN Xian1,ZHOU Jianjiang1,2 (1.The Key Laboratory of Molecular Biology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Department of Internal Medicine,Cancer Hospital of Guizhou Province,Guiyang 550004,Guizhou,China);Expression of Gastrin Gene in Human Gastric Cancer Tissues and Metastatic Lymphatic Tissues[J];贵阳医学院学报;2011-03
5 SHAN Chang-Liang,ZHANG Shuai,ZHANG Xiao-Dong,YE Li-Hong(Key Laboratory of Bioactive Material of Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China);Hepatitis B Virus X Protein Mutant (HBxΔ127) Promotes Hepatoma Cells Migration via ERK-induced Upregulation of Calpain Small Subunit 1[J];中国生物化学与分子生物学报;2011-05
6 CUI Hong-Xia1),2),YUE Li-Ling1),LIU Ji-Cheng1)*(1) Department of Pharmacology,Institute of Medicine,Qiqihar Medical College,Qiqihar 161006,China;2) Heilongjiang University of Chinese Medicine,Harbin 150040,China);Exogenetic Overexpression of ST3Gal Ⅰ Increases the Ability of Adhesion and Invasion to ECM in Breast Cancer MCF-7 Cells[J];中国生物化学与分子生物学报;2011-04
7 Michael D Burkitt, D Mark Pritchard, Gastroenterology Unit, School of Clinical Sciences, University of Liverpool, Ashton Street, Liverpool, L69 3GE, United Kingdom Andrea Varro, The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool, L69 3BX, United Kingdom;Importance of gastrin in the pathogenesis and treatment of gastric tumors[J];World Journal of Gastroenterology;2009-01
8 Jia-Ding Mao, Pei Wu, Ying-Lin Yang, Jian Wu, He Huang, Department of General Surgery, the First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui Province, China;Relationship between expression of gastrin, somatostatin,Fas/FasL and caspases in large intestinal carcinoma[J];World Journal of Gastroenterology;2008-18
9 ZHANG Shu-jie,WANG Rui-fen,WANG Zheng-cai,et al (Department of Pathology,The First Clinical College,Harbin Medical University,Harbin 150001,China);The correlation between the expressions of gastrin,somatostatin and the metastasis of lymph node in gastrointestinal carcinoid[J];哈尔滨医科大学学报;2007-04
10 Pei Wu, Jia-Ding Mao, Jing-Yi Yan, Jing Rui, You-Cai Zhao, Xian-Hai Li, Guo-Qiang Xu, Department of General Surgery, The First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui Province, China You-Cai Zhao, Guo-Qiang Xu, Department of Pathology, The First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui Province, China;Correlation between the expressions of gastrin, somatostatin and cyclin and cyclin-depend kinase in colorectal cancer[J];World Journal of Gastroenterology;2005-45
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