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《Chongqing Medicine》 2017-32
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Preparation of myocardial ischemia/reperfusion injury model by overlapping line in mice

Jia Zhongshen;Jiang Zhi;Li Anjie;Wu Yueting;Wei Fang;Department of Cardiology,the Affiliated People′s Hospital of Guizhou Medical University;  
Objective To improve the operation speed and reliability of the myocardial ischemia/reperfusion injury(I/R)model in mice.Methods The operative time and the survival rate of the three models were compared.The postoperative leukocyte count and serum IL-6 level were detected to evaluate the inflammatory response.The infarct size was assessed by TTC/Evans blue staining.The left ventricular end diastolic pressure and the pressure change rate were measured by apressure catheter for the evaluation of cardiac function.In traditional method,open chest surgery was performed to ligate and loose the left coronary artery(LCA)under the assistance of intubation and ventilation.In novel method,isoflurane was used for anesthesis,and then heart was squeezed out of the chest to ligate and loose LCA with a slipknot.In overlapping line method,the heart was also squeezed out,but a square knot was performed to ligate LCA and a 2-0 nylon together,and then the nylon thread was drew out to loose LCA.Results Compared with traditional method,the novel method and overlapping line method had significant short operation time(P0.01 or P0.05),less white blood cell count and serum IL-6 concentration(P0.05)and higher mice survival rate(P0.01);compared with novel method,the overlapping line method further simplified the reperfusion procedure and decreased total procedure time(P0.01).There was no significant difference in cardiac infarct size and cardiac function by using the three methods(P0.05),but the overlapping line method had less coefficient of variation compared to novel method.Conclusion Compared with the novel method,the overlapping line method reduces the operation difficulty,shortens the operation time and improves the stability of I/R model.
【Fund】: 国家自然科学基金地区科学基金资助项目(81460050);; 贵州省科技厅科学技术基金[黔科合J字(2014)2112号]
【CateGory Index】: R-332;R542.22
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