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《Journal of Changshu Institute of Technology》 2011-02
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Improvement in Educational Experiment of Esterase Isozyme Electrophoresis of Edible Fungi

YAO Lu-ye,DING Hai-yang,NI Xin-chen,ZHU Jing,SUN Feng-dan,JI Hong(School of Biological Science and Food Engineering,Changshu Institute of Technology,Changshu 215500,China)  
The experiment electrophoresis of esterase isozyme which was suggested in some material could not apply to the school laboratory conditions.In this paper,the measurement of esterase isozyme was improved from some aspects,such as casting gel,treatment samples and electrophoresis,while the whole experimentation was based on the smooth-type Pleurotus eryngi(called S-type)and rough-type Pleurotus eryngi(called R-type).Those procedures in isozyme electrophoresis were modified to make this technique more practicable,especially suitable to educational lab.Through improved electrophoresis,the results were performed to analyze the esterase isozymes of S-type and R-type during the cultivate time,and it was found that they had 6 and 4 characteristic bands,respectively.
【Fund】: 2010年江苏省大学生创新实践训练计划项目
【CateGory Index】: S646
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