Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Forest By-Product and Speciality in China》 2006-04
Add to Favorite Get Latest Update

Study on Extraction and Purification of Lentinan

Lu Chengying,Li Guozhang,Huang Zaocheng,Guo Xiaohui,Cao Yong (The Key Laboratory For Forest Products Chemical Industry Engineering of Hunan,Ji ShouUniversity;Zhangjiajie,Hunan 427000)  
Using 0.2mol/L CCl_3COOH,0.4 mol/L NaOH,1.0 mol/L CH_3COOH,water as solvent,polysaccharide was extracted from Lentinus edodes.After 50% methyl alcohol eliminate impurity and 75% methyl alcohol precipitate Lentinan crude.Then drying the Lentinan crude,using water to dissolve it,and adding 0.15mol/L CTAB of same volume to precipitate Lentinan,precipitate was added 2mol/L acetic acid to dissolve Lentinan and got rid of the infusible thing.Using 50% methyl alcohol to eliminate impurity in the filtrate, then adding 75% methyl alcohol to precipitate Lentinan again.Purity of the Lentinan extracted by 0.2 mol/L of CCl_3COOH is up to 93.1%,the extraction rate is 3.24‰.TLC showed that the Lentinan contain glucose 、 mannose、galactose and xylose.sephadex-100 column chromatography and vitriol-phenol method suggested that Lentinan has one peak,IR spectrum suggested that Lentinan has a chart,which looks the same to that of amylose.
【CateGory Index】: TQ464.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 LI Jun1,ZHONG Yao-guang1,2,LIU Chang-jiang2(1.College of Food Science and Technology,Shanghai Fisheries University,Shanghai 200090,China;2.College of Food Science,Shenyang Agricultural University,Shenyang,Liaoning 110161,China);Advances on Methods for Extraction and Purification of Lentinan[J];Northern Horticulture;2008-12
2 GUO Zhen-huan~1,HU Yuan-liang~(1*),MA Xia~1,ZHAO Xiao-na~1,WANG De-yun~1, ZHAO Bing-kai~1,GUO Li-wei~1,LIU Ping~1,Hao Li-hua~2 (1.Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China; 2.China Institute of Veterinary Drug Control,Beijing 100081,China);Adjuvanticity of sulfated lentinan for Newcastle disease vaccine[J];Journal of Nanjing Agricultural University;2010-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 TANG Li Qin, LI Chu, LIU Sheng, CHEN Li Ming (Dept of Pharmacy, Anhui provincial hospital, Hefei 230001);Determination of Pachyman in Radix Ophiogonis content by Anthrone-Sulphuric Acid Colrimetry[J];Anhui Medical and Pharmaceutical Journal;2003-01
2 Dong Airong(Shenyang Agricultural University, Shenyang 110161, P. R. China); Yu Qingyu, He Li(Northeast Forestry University); Wang Wenge, Pang Lijie(the Administrative Bureau of State-owned Forest Farm of Shangzhi City, Heilongjiang Province); Hao Chongxi;Biological Character of Morchella esculenta[J];Journal of Northeast Forestry University;2002-04
3 Zhang Xiaohua Ren Chengang Guo Shuntang(The Protein Processing and Utility Laboratory of Food Science and Nutritional EngineeringCollege of China Agricultural University,Beijing,100083);THE EXTRACTION AND UTILIZATION OF SOLUBLE SOYBEAN POLYSACCHARIDE[J];Soybean Science;2006-01
4 TIAN Chun-yu1,WANG Guan-lin21.School of Environmental and Biological Science and Technology,Dalian University of Technology,Dalian 116024,China;2.College of Life Science,Liaoning Normal University,Dalian 116029,China;Isolation,purification and physical-chemical properties of polysaccharides from sweet potatoes[J];Journal of Dalian University of Technology;2009-01
5 GAO Bin,GAO Hong (College of Animals Science and Technology ,Yunnan Agricultural University,Kunming,Yunnan,650201, China);Oxygen Free Radical and Cell Damage[J];Progress In Veterinary Medicine;2002-05
6 Wan Zhan; Fang Jinian(Shanghai Institute of Materica Medica,Academy of Sciences,Shanghai 200031);Application of Nuclear Magnetic Resonance Spectroscopy in the Structural Determination of Polysaccharide[J];CHINESE JOURNAL OF ANALYTIEAL CHEMISTRY;2000-02
7 Li Yan,Wang Zhaomei,Guo Siyuan(Light Industry and Chemical Engineering Research Institute,South China University of Technology,Guangzhou 510640,China);Review on the Advanced Structure of Bioactive Polysaccharides[J];Guangdong Chemical Industry;2005-08
8 ZHANG Li Na *, ZHANG Ping Yi, LI Xiang, PAN Su (Department of Chemistry, Wuhan University, Wuhan, 430072) ZHU Rong Ping (National Research Center for Certified Reference Materials, Beijing, 100013);Studies on Components and Molecular Weights of Polysaccharides from Lentinus Edodes[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1998-09
9 Unursaikhan Surenjav~a Li-na Zhang~a(**) Xiao-juan Xu~a Mei Zhang~a Peter Chi Keung Cheung~b and Fan-bo Zeng~c ~aDepartment of Chemistry,Wuhan University,Wuhan 430072,China ~bDepartment of Biological,The Chinese University,of Hong Kong,Hong Kong,China ~cTongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;STRUCTURE,MOLECULAR WEIGHT AND BIOACTIVITIES OF(1→3)-β-D-GLUCANS AND ITS SULFATED DERIVATIVES FROM FOUR KINDS OF LENTINUS EDODES[J];高分子科学(英文版);2005-03
10 DONG Cai-Xia HUANG Jian-Hua ZHU Yong HAN Jian-Zhi (Department of Chemical Engineering,Henan Institute of Science and Technology,Xinxiang,Henan 453003,P.R.China);Extraction of Polysacchride in Lentinus Edodes by Enzyme-Decomposition[J];Chinese Journal of Spectroscopy Laboratory;2005-05
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 Zhu Deyan(Jingchu University of Technology,Jingmen,Hubei 448000,China);Comparison of Some Ways of Purifying Lentinan[J];;2009-04
2 Tang Ming,Qin Dan,Liu Sha(College of Food Science,Hu'nan Agricultural University,Changsha,Hu'nan 410128,China);Research Advance on Extraction and Purification Methods of Polysaccharide[J];;2009-10
3 LI Xiang-yang1,HU Yi-hong1,WEI Xiang-hua2,ZENG Yong-xian1 (1. Department of Life Sciences,Hunan Institute of Humanity and Technology,Loudi 417000,PRC; 2. College of Agronomy,Guangxi University,Nanning 530004,PRC);Comparison of Antioxidative and Antimicrobial Abilities of Lentinan between Wood Planting Flower-mushroom and Bag Planting Flower-mushroom[J];Hunan Agricultural Sciences;2009-11
4 SHEN Jin-wen 1,FENG Ya-lan 2,ZHUANG Qing-li 1,YU Hai-you 1,ZHANG Biao 1,WANG Zhen 1,MA Bing-Ji 3 (1.College of Life Science,Henan Agricultural University,Zhengzhou 450002,China;2.Xinyang Institute of Agricultural Science,Xinyang 464000,China;3.College of Agriculture,Henan Agricultural University,Zhengzhou 450002,China);Optimization on the Extraction Technology of Polysaccharides from Sporocarp of Lentinus edodes[J];Journal of Henan Agricultural Sciences;2010-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 FANG JI-NIAN YE CHUN-QU LIU YU-RONG(Shanghai Institute of Materia Medica,Academia Sinica);CHEMICAL INVESTIGATION OF NEW POLYSACCH ARIDES FROM SHIRAIA BAMBUSICOLA[J];Acta Chimica Sinica;1982-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved