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《Forest By-Product and Speciality in China》 2006-04
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Study on Extraction and Purification of Lentinan

Lu Chengying,Li Guozhang,Huang Zaocheng,Guo Xiaohui,Cao Yong (The Key Laboratory For Forest Products Chemical Industry Engineering of Hunan,Ji ShouUniversity;Zhangjiajie,Hunan 427000)  
Using 0.2mol/L CCl_3COOH,0.4 mol/L NaOH,1.0 mol/L CH_3COOH,water as solvent,polysaccharide was extracted from Lentinus edodes.After 50% methyl alcohol eliminate impurity and 75% methyl alcohol precipitate Lentinan crude.Then drying the Lentinan crude,using water to dissolve it,and adding 0.15mol/L CTAB of same volume to precipitate Lentinan,precipitate was added 2mol/L acetic acid to dissolve Lentinan and got rid of the infusible thing.Using 50% methyl alcohol to eliminate impurity in the filtrate, then adding 75% methyl alcohol to precipitate Lentinan again.Purity of the Lentinan extracted by 0.2 mol/L of CCl_3COOH is up to 93.1%,the extraction rate is 3.24‰.TLC showed that the Lentinan contain glucose 、 mannose、galactose and xylose.sephadex-100 column chromatography and vitriol-phenol method suggested that Lentinan has one peak,IR spectrum suggested that Lentinan has a chart,which looks the same to that of amylose.
【CateGory Index】: TQ464.1
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Chinese Journal Full-text Database 2 Hits
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7 Li Yan,Wang Zhaomei,Guo Siyuan(Light Industry and Chemical Engineering Research Institute,South China University of Technology,Guangzhou 510640,China);Review on the Advanced Structure of Bioactive Polysaccharides[J];Guangdong Chemical Industry;2005-08
8 ZHANG Li Na *, ZHANG Ping Yi, LI Xiang, PAN Su (Department of Chemistry, Wuhan University, Wuhan, 430072) ZHU Rong Ping (National Research Center for Certified Reference Materials, Beijing, 100013);Studies on Components and Molecular Weights of Polysaccharides from Lentinus Edodes[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1998-09
9 Unursaikhan Surenjav~a Li-na Zhang~a(**) Xiao-juan Xu~a Mei Zhang~a Peter Chi Keung Cheung~b and Fan-bo Zeng~c ~aDepartment of Chemistry,Wuhan University,Wuhan 430072,China ~bDepartment of Biological,The Chinese University,of Hong Kong,Hong Kong,China ~cTongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;STRUCTURE,MOLECULAR WEIGHT AND BIOACTIVITIES OF(1→3)-β-D-GLUCANS AND ITS SULFATED DERIVATIVES FROM FOUR KINDS OF LENTINUS EDODES[J];高分子科学(英文版);2005-03
10 DONG Cai-Xia HUANG Jian-Hua ZHU Yong HAN Jian-Zhi (Department of Chemical Engineering,Henan Institute of Science and Technology,Xinxiang,Henan 453003,P.R.China);Extraction of Polysacchride in Lentinus Edodes by Enzyme-Decomposition[J];Chinese Journal of Spectroscopy Laboratory;2005-05
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 Zhu Deyan(Jingchu University of Technology,Jingmen,Hubei 448000,China);Comparison of Some Ways of Purifying Lentinan[J];;2009-04
2 Tang Ming,Qin Dan,Liu Sha(College of Food Science,Hu'nan Agricultural University,Changsha,Hu'nan 410128,China);Research Advance on Extraction and Purification Methods of Polysaccharide[J];;2009-10
3 LI Xiang-yang1,HU Yi-hong1,WEI Xiang-hua2,ZENG Yong-xian1 (1. Department of Life Sciences,Hunan Institute of Humanity and Technology,Loudi 417000,PRC; 2. College of Agronomy,Guangxi University,Nanning 530004,PRC);Comparison of Antioxidative and Antimicrobial Abilities of Lentinan between Wood Planting Flower-mushroom and Bag Planting Flower-mushroom[J];Hunan Agricultural Sciences;2009-11
4 SHEN Jin-wen 1,FENG Ya-lan 2,ZHUANG Qing-li 1,YU Hai-you 1,ZHANG Biao 1,WANG Zhen 1,MA Bing-Ji 3 (1.College of Life Science,Henan Agricultural University,Zhengzhou 450002,China;2.Xinyang Institute of Agricultural Science,Xinyang 464000,China;3.College of Agriculture,Henan Agricultural University,Zhengzhou 450002,China);Optimization on the Extraction Technology of Polysaccharides from Sporocarp of Lentinus edodes[J];Journal of Henan Agricultural Sciences;2010-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
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