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《Forest By-Product and Speciality in China》 2008-04
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Analysis of Genetic Diversity and Affinity Relationships Among the Medical Species of Dendrobium Sw.by RAPD

Qiu Jing1,Fan Honghong1,Li Tingchun2,Lin Yi1,Cai Yongping1(1.School of Life Science,Anhui Agriculture University,Heifei 230036 2.Anhui Provincial Tobacco Research Institute,Fengyang,Anhui 233100)  
Genetic diversity and affinity relationships among 9 medical species of Dendrobium Sw.were analyzed,the result laid a solid foundation for the better use of this resources.The genetic diversity of 9 species Dendrobium Sw.was tested by using RAPD(random amplified polymorphic DNA) technique.The NTSYS software was used to analyze the markers.Seventy RAPD primers from 103 random primers were applied to do random amplification.A total of 520 DNA bands was detected,471 among which were polymorphic,the average rate of polymorphic bands was 94.42%.The result of cluster analysis by using UPGMA method showed that 9 genotypes could be classified into two types in genetic distance 0.44.This outcome was corresponding to the result by using traditional classification.Conclusion: The result of this research showed that RAPD molecular marker is efficient to study the medical Dendrobium Sw.genetic diversity.
【Fund】: 安徽省教育厅自然科学重点科研项目(2006KJ054A)
【CateGory Index】: S567.239
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