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《JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI》 1997-01
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Production and Properties of Inulinase From Aspergillus niger M_(89)

Jia Yingmin(Dept. of Food Science,Hebei Agric. Univ.,Baoding 071001 )Zhao Xuehui(Dept. of Food Science and Technology,Hua Zhong Agric. Univ-,Wuhan 430070)  
Inulinase yielding related factors of strain Aspergillus niger M,, were studied.The strain gave the highest enzyme levels with inulin as catbon source. The optimalnitrogen substrates for inulinase production of the strain were ammonium oxalate,(NH4)2SO4 and NH4H2PO4. Aspergillus niger M,,produced maximal inulinase activity withthe medium initial pH7. 0 at temperature 32℃ 210r/min Stirring culture. The optimaltemperatrue for the inulinase activity was between 55~60℃ with pH4. 5. Incubated underthe condition of 60℃ for 3h,the inulinase activity Iosed only 17%. At room temperature,dialyzed with pH4. 0~9.0 buffer for 12h,Inuliuase activity could be recovered by 100%'
【Fund】: 河北省自然科学基金
【CateGory Index】: Q55
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