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《Journal of Agricultural University of Hebei》 1997-01
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Experiment of in Vitro Fertilization of Goat Small Antral Folliculat Oocytes

Zhong Yong ;Liu Sujuon ;Li Yuqong; Rui Rong; Li yong(Labratory of Deuelopment Biology Northwestern Agricultural University. YangLing 712100 )  
After the follicles of 2 ~ 6mm (in diameter) were aspirated, the goat ovariantissue was dissected lengthwise and crosswise with the device of four razors arrangedprallelly at 1. 5mm intervals to recover2mm small antral follicular oocytes. The diameterof small antra1 follicular oocytes was measured. The result was 129. 86± 18. 84μm. 218oocytes with the compact and dense cumulus cells and homogeneous ooplasm were culturedin 20mM HEPES buffered;①TCM199 + 15 %GS, ②①+ FSH 、LH (10μ/mL ) + 17β- E2(1μg/mL ), ③② + LTS (5μg/ml, Insulin, Transferrin, 5ng/mI, Sodium Selenite ), ④TCM 199 + 15 %NCS + FSH 、 LH + 17β - E2 for 26 ~ 28hs, and each medium had granu1osacells. The maturation rate were 19. 35 % (6/31 ), 26. 53 % (26/98), 29. 45 % (43/146 ) and20. 75 % (1l/53 )respectively- The matured ova were incubated with capacitated spermsfor insemination. The fertilization rate was 32. 94% (28/85 ) and the rate of 2 - celldevelopment was 7. 14 % (2/28).
【Fund】: 国家自然科学基金;;农业部生物技术攻关项目
【CateGory Index】: S827.3
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【References】
Chinese Journal Full-text Database 2 Hits
1 ZHENG Pei-dong~1,2,WANG Xin-zhuang~2,1,CHEN Jiang-ling~1,WANG Ju-qiang~2,XIAO Jie~2(1.College of Husbandry and Veterinary Medicine Engineering,Henan Agricultural University,Zhengzhou 450002,China;2.The Research Center of Beef Engineering,Zhengzhou 450003,China);Study on in Vitro Maturation of Nanyang Cattle Oocytes Derived from Small Antral Follicles[J];Journal of Henan Agricultural Sciences;2006-10
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1 TUNG, T. C., Y. F. Y. TUNG, T. Y. LUH, S. M. TUNG AND M, TU(Institute of Zoology and Institute of Oceanoloay, Academia Sinica);TRANSPLANTATION OF NUCLEI BETWEEN TWO SUBFAMILIES OF TELEOSTS (GOLDFISH——DOMESTICATED CARASS1US AURATUS,AND CHINESE BITTERLING——RHODEUS SINENSIS)[J];Acta Zoologica Sinica;1973-03
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