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《Journal of Agricultural University of Hebei》 2003-04
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The study of salt-tolerance ability of seedling and callus in wild soybean

QIAO Yake,LI Guilan,WANG Wenpo,GAO Shuguo,BI Yanjuan (Hebei Vocation-technical Teachers College, Changli 066600, China)  
The 14 genotypes of wild soybean growing at different condition in eastern of Hebei province were studied in this experiment. Hypocotyls and cotyledon were used as explants, callus was induced on medium containing different composition. The salt-tolerance of callus was observed at different medium containing 0,02%,04%, 06%,08%,10% NaCl. Ability of salt-tolerance was identified through growthamount,quality,becoming brown and death ratio of callus. The results showed that MS+20 mg/L 2,4-D+05 mg/L 6-BA+sucrose 5% +agar 08% is the best medium for callus induction. Ability of salt-tolerance of callus had obvious difference among the different genotypes. The stronger salt-tolerance genotypes could survive at the concentration of 08% NaCl. The ability of salt-tolerance of 6,7,10,11,12,14 was stronger, the growth amount and quality of callus descended with the rising of concentration of NaCl. The experiment indicated that identity indicator of salt-tolerance of wild soybean is 06% NaCl. Generally, the wild soybean from saline soil have stronger ability of salt-tolerance. The results suggested that the relation of salt-tolerance ability at cell level and plant level should study further.
【Fund】: 河北省科技厅资助项目(99230313)
【CateGory Index】: S565.1
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