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《Journal of Agricultural University of Hebei》 2004-03
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BAC library construction of Zhongmiansuo 12 with high yield, high quality and disease resistance

ZHENG Yong-min,WANG Xing-fen,ZHANG Gui-yin,LI Xi-huan,MA Zhi-ying sources of Hebei, College of Agriculture, Agricultural University of Hebei, Baoding 071001, China)  
The bacterial artificial chromosome(BAC) library of Zhongmiansuo 12, a Chinese elite cotton variety with high yield, high quality and disease resistance, was constructed based on plndigoBAC-5 vector. The library included 38 800 clones and was two haploid genome equivalents based on a AD genome size of 2 240 Mb. Analysis results of 132 recombinants indicated that the insert DNA size ranged from 50 to 150 kb, averaged 120 kb. As much as 87.7% clones had inserts over 100 kb, and 56% clones over 110 kb. The empty clone was less than 1% in the library. This library provided a useful tool for the cotton genome research.
【Fund】: 国家自然科学基金(30270848);; 教育部重点科技项目(204017)
【CateGory Index】: S562
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