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《Journal of Agricultural University of Hebei》 2004-04
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The study of three streptomycetes-phage

ZHANG Xiu-min,XIONG Ya-nan,CHAO Wei-feng,ZHANG Li-ping(College of Life Science, Hebei University, Baoding 071002, China)  
Three strains of streptomyces-phages (HPA1, HPA2, HPA3) were isolated from soil samples collected from Baoding, China. Their morphology and stability were studied. The morphologies of their plaques are different in nutrient agar medium. The plaque's size of HPA1 is medium and transparent. The plaque of HPA2 has transparent center with obscure edge ,which is bigger than others and similar as solar halo. The last type of PHA3 is small and like pin prick. The result of electronic microscope showed that external forms of their heads are all circular shape, but the sizes and length of their tails varied. HPA1 has long tail, and HPA3 has short tail, while HPA2 has no tail . Their host range is narrow. They are unable to infect Streptosporangium strains and other actinomycetes, expect some streptomyces species. Among the three streptomyces-phages (HPA1, HPA2, HPA3) , HPA2 is more tolerate to temperature than others. When temperature reached 60℃, 21% of HPA2 were still survived. HPA1 and HPA3 are sensitive to chlorotorm,while HPA2 is resistant to chlorotorm. In addition, they are all sensitive to ultraviolet ray. When they were exposed to ultraviolet ray for 5 minutes, their rates of Plaque-forming were outstandingly decreased. They all have obvious resistance to potassium dichromate and actidione.
【CateGory Index】: Q93
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