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《Journal of Agricultural University of Hebei》 2005-03
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Establishment of SRAP technique system in wheat genome

WU Zhi-pu,YANG Wen-xiang,LIU Da-qun,ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, China)  
In this paper, some factors involving genomic DNA template, dNTP Mixture, Taq polymerase and PCR amplification time and temperatures of denature, annealing and amplification affecting the SRAP researching system of wheat were studied and an advanced technical system was established. The 20 μL reaction mixture contained: 25 ng of genomic DNA template, 188 mmol/L of dNTP, 0.75 U of Taq polymerase, 25 ng of primer and appropriate reaction buffer. Amplification was carried out in two steps: in the first 5 cycles, the annealing temperature was 37℃, and during the following 35 cycles, the annealing temperature was 50℃. In all the cycles, denaturation was carried out for 1 min at 94℃, annealing for 1 min, and amplification for 10 sec at 72℃. After all the cycles, amplification was done for 1 min at 72℃.
【Fund】: 国家自然科学基金(30170602)赞助项目
【CateGory Index】: S512.1
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Chinese Journal Full-text Database 10 Hits
1 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
2 RUAN Xian-le et al (Department of Life Science,Zhoukou Normal University,Zhoukou,Henan 466000);Optimization of SRAP-PCR Reaction System in Potato[J];Journal of Anhui Agricultural Sciences;2009-25
3 Liu Lijun Meng Zuqing Xing Xiulong Peng Dingxiang* College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070;Optimization for SRAP Reaction System in Ramie (Boehmeria nivea L. Guad)[J];Molecular Plant Breeding;2006-05
4 Chen Daxia Li Longyun Li Quansen Qin Songyun Zhong Guoyue Chongqing Academy of Chinese Materia Medic.a,Chongqing,400065;Optimization of SRAP-PCR System for Curculigo L.Plant[J];Molecular Plant Breeding;2007-S1
5 LIN Qiang1,QIU Chang-yu1,ZHU Fang-rong1,ZHAO Wei-guo2,CHEN Xiao-qing1,HUANG Xu-hua1,ZHU Guang-shu1,LI Yi1,ZENG Yan-rong1(1 Guangxi Sericulture Technology Extension Station,Nanning 530007,China;2 Sericulture Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang,Jiangsu 212018,China);Optimization of SRAP-PCR method for mulberry[J];Guangxi Agricultural Sciences;2010-11
6 LIU Li-Hua~1,MA Xiao-Jun~1,SUN Jing-Jing~2, QIN Jia-Ming~3,WEI Jian-He~1 (1.Institute of Medicinal Plant Development,China Academy Medicinal Science,Chinese Peking Union Medical Colledge,Beijing 100193,China;2.College of Science,Liaoning Technical University,Fuxin 123000, China;3.Guangxi Maize Research Institute,Nanning 530227,China);Establishment and optimization of SRAP reaction system in Siraitia grosvenorii[J];Guihaia;2009-06
7 ZHOU Chun-E,GU Feng-Ping,LU Shu-Xia,DUAN Hong-Ying,ZHOU Yan-Qing( College of Life Science,Henan Normal University,Xinxiang 453007,China );Establishment of SRAP amplification system for Rehmannia glutinosa and primer screening[J];Guihaia;2010-02
8 LI Xiao-hui1,WANG Cong-yan2,XU Xiao-li1,CHANG Gao-zheng1,ZHANG Si-pu1(1.Institute of Horticulture,Henan Academy of Agriculture Sciences,Zhengzhou 450002,China;2.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China);The Optimization of SRAP-PCR Amplification Program and System for Citrullus lanatus[J];Acta Agriculturae Boreali-Sinica;2008-03
9 YAO Jian-hua1,FU Hong-tuo1,2,GONG Yong-sheng2,WU Yan2,HE Xin-long2,LI Hui-ping1,QIAO Hui1(1.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;2.Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China);Orthogonal Design and Optimization of SRAP Amplification System for Macrobrachium nipponense[J];Acta Agriculturae Boreali-Sinica;2008-S2
10 SHI Hong-li,HAN Ming-yu,ZHAO Cai-ping(College of Horticulture,Northwest Agricultural and Forestry University,Yangling 712100,China);Optimization of SRAP Reaction System in Peach(Prunus persica)[J];Acta Agriculturae Boreali-Sinica;2008-S2
Chinese Journal Full-text Database 2 Hits
1 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
2 PAN Qing-hua, HU Tie-zhu, WANG Ling, CAI Jiang-qiao (Laboratory of Plant Resistance and Genetics, College of Natural Resource Environment, South China Agricultural University, Guangzhou 510642);Molecular Genetic Studies on the Rice Blast Fungus Population DNA fingerprinting and pathotype analyses of the isolates selected from the region-specific lineages of Guangdong Province, China[J];Scientia Agricultura Sinica;2004-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Jing,HU Xiao-hui,MIAO Hua-rong,ZHANG Jian-cheng,YU Shan-lin(Shandong Peanut Research Institute,Qingdao 266100,China);Optimization of SRAP-PCR Reaction System on Peanut[J];Shandong Agricultural Sciences;2008-05
2 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
3 GUO Da-long et al(College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003);Establishment and Optimization of SRAP Amplification System in Bitter Melon(Momordica charantial L.)[J];Journal of Anhui Agricultural Sciences;2008-18
4 XIANG Zhong-ping et al(School of Civil Engineering,Jiujiang College,Jiujiang,Jiangxi 332005);Research Progress of Lycoris Herb Plant[J];Journal of Anhui Agricultural Sciences;2010-03
5 TIAN Jing-wen (Nationality Vocational College of Southeast Guizhou,Kaili,Guizhou 556000);Research on the Adaptability of Peach in Kaili Region and Its Cultivation Technique[J];Journal of Anhui Agricultural Sciences;2010-22
6 LI Yue-xian et al (Tropical and Subtropical Cash Crops Institute of Yunnan,Agriculture Science Research Institute,Baoshan,Yunan 678025);Research Summary and Future Prospects of Molecular Marker Technology in Cassava[J];Journal of Anhui Agricultural Sciences;2011-02
7 DENG Bo-tao ZHAO Fei WANG Xue-ying (College of Biological Sciences and Technology,Shenyang Agricultural University,Shenyang Liaoning 110161);Optimization of SRAP in Carrot[J];;2009-13
8 ZHANG Ting LIU Shuang-qing LV Ming-zhi(Department of Biotechnology,Wuhan Bioengineering Institute,Wuhan Hubei 430415);Applications of Sequence-Related Amplified Polymorphism(SRAP) Markers in Crop Heredity and Breeding[J];Modern Agricultural Sciences and Technology;2010-11
9 XU Ao-jing,ZHU Li-wu,JIA Bing,HENG Wei,YE Zhen-feng(Key Laboratory of Pomology,Anhui Agricultural University,Hefei 230036);Primarily identification of pear variants and analysis of their genetic relationship by SRAP markers[J];Journal of Anhui Agricultural University;2011-03
10 WU Zhi-ming1,ZENG Jin2,HU Kai-lin2,QIAO Ai-min1(1.College of Horticulture and Landscape Architecture,Zhongkai University of Agricultural and Engineering,Guangzhou,Guangdong 510225;2.College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510640);Optimization of SRAP-PCR System for Pepper Using Orthogonal Design[J];Northern Horticulture;2010-11
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Zuo Kaijing Sun Jizhong Zhang Xianlong Nie Yichun Liu Jinlan Feng Chunda(National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University, Wuhan 430070);Constructing a Linkage Map of Upland Cotton ( Gossypium hirsutum L.) Using RFLP, RAPD and SSR Markers[J];JOURNAL OF HUAZHONG AGRICULTURAL;2000-03
2 DENG Xiao-Ling AN Shu-Wen LIU Si-Guo PAN Qing-Hua (Laboratory of Plant Resistance and Genetics, College of Natural Resource &Environment, South China Agricutural University, Guangzhou,510642);MOLECULAR GENETIC STUDIES ON THE RICE BLAST FUNGUS POPULATION I. GENETIC DIVERSITY OF THE RICE BLAST FUNGUS POPULATION IN CHINESE GUANGDONG: 1998~1999[J];Mycosystema;2002-01
3 Shen Ying; Yuan Xiaoping; Wang Yanli (China National Rice Research Institute, Hangzhou 310006, China);MOLECULAR PROBES FOR EPIDEMIOLOGICAL STUDIES OF THE RICE BLAST FUNGUS[J];JOURNAL OF SOUTHWEST AGRICULTURAL UNIVERSITY;1998-05
4 Ling Zhongzhuan 1,T.Mew 2,Wang Jiulin 1,Lei Cailin 1,Huang Ning 2 ( 1Institute of Crop Breeding and Cultivation, CAAS, Beijing 100081; 2International Rice Research Institute);Development of Chinese Near isogenic Lines of Rice and Their Differentiating Ability to Pathogenic Races ofPyricularia grisea[J];SCIENTIA AGRICULTURA SINICA;2000-04
5 HU Tie-zhu, WANG Ling, FENG Xi-lu, PAN Qing-hua (Laboratory of Plant Resistance and Genetics, College of Natu ral Resource and Environment, South China Agricultural University, Guangzhou 5 10642);Molecular Genetic Studies on the Rice Blast Fungus Population——Genetic Structure of the Rice Blast Fungus Population Consisted of Five Sub-populations Selected from Five Counties in Guangdong Province, China[J];Scientia Agricultura Sinica;2003-12
6 All China Coorporation of Research on Physiological Races of Pyricularia oryzae;RESEARCH ON PHYSIOLOGICAL RACES OF RICE BLAST FUNGUS IN CHINA[J];Acta Phytopathologica Sinica;1980-02
7 Wu Shangzhong;Luo Lin; Zhang Shaohong; Yang Qiyun; Zhu Xiaoyuan; Liu Bin (Plant Protection Institute of Guangdong Academy of Agricultural Sciences, Guangzhou 510640)(Guangdong Key Laboratory of Cell Engineering in Rice Breeding of Guangdong Academy of Agr;FINGERPRINTING ANALYSIS OF MAGNAPORTHE GRISEA IN GUANGDONG PROVINCE AND THEIR GENETIC LINEAGE STRUCTURE[J];ACTA PHYTOPATHOLOGICA SINICA;1998-04
8 LEI Cai lin 1, WANG Jiu lin 1, JIANG Wan ru 1, LING Zhong zhuan 1,M.L.GEORGE 2 (1 Institute of Crop Breeding and Cultivation, CAAS, Beijing 100081,China; 2 International Rice Research Institute, Makati, Philippines);POPULATION STRUCTURE AND GENETIC VARIATION OF RICE BLAST FUNGUS IN SOME RICE-GROWING REGIONS IN NORTHERN CHINA[J];Acta Phytopathologica Sinica;2002-03
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