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《Journal of Agricultural University of Hebei》 2005-05
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Studies on insect-resistant mechanism of transgenic triploid of Chinese white poplar

YUAN Sheng-liang~1,GAO Fei~2,ZHOU Guo-na~1,LI Ming~1ZHAO Yong-qiang~1,GAO Bao-jia~1(1.College of the Forestry,Agricultural University of Hebei,Baoding 071000,China;2.College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China)  
The activities of detoxification enzymes and digestive enzymes of Clostera anachoreta(Fabricius) instar larvae feeding on leaves of three kinds of insect-resistant clones of transgenic Triploid of Chinese White Poplar,after 4,12,24,48,72 and 96 hours,were investigated.The results showed that,feeding on the clone 7,the activity of esterase,carboxylesterase and mix-function oxidase in the midgut of the larvae were tremendously decreased.Feeding on the clone 10,those results were less than the results above,and there were few changes on the larvaes which feeded on the clone 26.Moreover,the changes of the amylase in the midgut of larvaes were the same as above.However,the activities of glutathione s-transferase and proteinases were complex,increased remarkably after 24 hours feeding on the clone 7,then declined rapidly.Same changes were taken place on the larvaes feeding on the clone 10.There are many slight changes on glutathione s-transferase of the larvaes,feeding on the clone 26,no changes on the proteinases in the midgut.Thus the detoxification enzymes and digestive enzymes in the midgut of the larvaes were inhibited.This may be the main mechanisms of the transgenic Triploid of Chinese White Poplar.
【CateGory Index】: S792.117
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