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Isolation and preliminary identification of the pathogen of Green Diamond anthracnose disease

FAN Yong-shan~1,LIU Hai-Ying~2,ZHANG Liang~1,HAN Xiao~1 (1.Tangshan Teacher's College,Tangshan 063000,China;2.Tangshan Agricultural Institute,Tangshan 063001,China)  
The description of symptom,the separation of pathogenic fungus and the pure culture about anthracnose were accomplished at the plant of Green Diamond in the flower market of Tangshan.The septate hypha was found at the PDA culture medium.The conidium generated on the conidiophores of the acervulus or accumulated was sticky orange spore groups directly on the colony.The brown setae could be found on the acervulus sometimes.The conidiophores had no color and parallelly gathered on the acervulus.Produced on the tip of conidiophore,the conidium was of long oval type.Its two ends were blunt with one end a little sharp.The condium was a single cell with no color and included two oil balls.The width and the length of the conidium were about 5.0~7.5 μm and 15.0~20.0 μm respectively.Therefore,the pathogen was preliminary identified to be Colletotrichum gloeosporioides Penz,and the definite species of the pathogen would be confirmed later.The pathogen was re-isolated from the diseased Green Diamond leaves after the in vivo inoculation of the fungus.
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