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《Journal of Agricultural University of Hebei》 2006-01
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Comparison between extracting methods for genomic DNA of dry leaf of Ziziphus Mill.saved in silica

LI Xue-ying,PENG Jian-ying,PENG Shi-qi(College of Horticulture,Agricultural University of Hebei,Baoding 071001,China)  
Improved CTAB,high salt precipitation,and high salt and low pH method were employed to extract genomic DNA from the leaves of 3 species of Ziziphus Mill.saved in silica for 15 days and for half a year.The extracted DNA was tested using the value of A_(260)/A_(280),agarose gel electrophoresis and PCR amplification.The DNA from the leaves saved in silica for half a year is good for PCR amplification and the method of high salt precipitation is best.
【Fund】: 国家自然科学基金资助项目(30270927);; 河北省自然科学基金项目(C2004000363)
【CateGory Index】: S665.1
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