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《Journal of Agricultural University of Hebei》 2006-02
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Genomic DNA extraction and optimization of RAPD analytic conditions of Michelia wilsonii

ZHU Xiu-zhi~1,XIANG Cheng-hua~2,PENG Zheng-song~1,YANG Jun-bao~1,LUO Cheng-ke~3 (1.College of Life Science,China West Normal University,Nanchong 637002,China;2.Sichuan Academy of Forestry,Chengdu 610000,China;3.Ningxia University,Yinchuan 750021,China)  
The method of genomic DNA extraction and the optimization of RAPD analytic conditions were studied in Michelia wilsonii.The results showed that high grade genomic DNA was obtained by the revised CTAB method.The optimal PCR system for RAPD analysis was as follows: 1.5 mmol/L Mg~(2+),0.8 mmol/L dNTP,240 nmol/L random primer,80 ng DNA template,1.0 U Taq polymerase,in 25 μL reaction system.
【Key Words】： Michelia wilsonii DNA extraction RAPD optimization
【Fund】： 四川省重点学科建设项目(SZD0420)
【CateGory Index】： S792.99
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