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Studies on the concentration of RBSDV in corn inbred lines with different resistance to the disease

ZHANG Ai-hong1,MIAO Hong-qin2,ZHU Bao-cheng1,LU Yin-gui2,CHEN Dan2,DI Dian-ping2(1.College of Life Science,Hebei University,Baoding 071000,China;2.Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,Hebei Engineering Research Centre for Integrated Control of Agricultural Pests,Baoding 071000,China)  
According to the height of the plants and symptoms on leaves,a criterion of 0-4 to examine the disease severity was reported previously.In this study,the criterion was adopted to grade the disease severity of the tested corn inbred lines with different resistance to the disease during the milky growth stage.Indirect enzyme-linked immunosorbent assay(ID-ELISA) was applied to detect rice black-streaked dwarf fijivirus(RBSDV) in newly sprout leaves.Variance analysis of the O.D405 values showed that there were significant differences on RBSDV concentrations among the different graded plants of the same inbred line.RBSDV concentrations were higher as the disease grading went up from 1 to 4 grades(F=237.499,P0.01).While in same disease grade of the resistant and susceptible inbred lines,there was no obvious difference on RBSDV concentration(F=0.775,P=0.598).This indicated that RBSDV concentration in leaves was positively related to the symptoms of whole plants;RBSDV could multiply in corn inbred lines with different resistance and move to the new growth leaves.
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