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《Journal of Agricultural University of Hebei》 2007-06
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Observation on chromosomes of European blackberry

XUE Chen-xin1,CUI Ming-wen2,ZHAO Jin3, LIU Meng-jun1 (1.Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding 071001,China;2.Math and Science Department,Hengshui University,Hengshui 053000,China;3.College of Life Science,Agricultural University of Hebei,Baoding 071001,China)  
Root tips and stem tips of blackberry tissue culture plantlet were used to observe the chromosomes number of European blackberry 'Thornfree'(Rubus fruticosus var.'Thornfree') by following the wall degradation hypotonic staining method.The main treatment parameters suitable for the observation of the chromosomes number of the blackberry were identified: the pre-disposal treatment time of mixed liquor of 0.02% colchicines and 2 mmol/L 8-hydroxyquino-line(1∶1) 2 h,the enzymolysis time of 2.5% cellulase and 2.5% pectase(1∶1) 2 h and the post-hypotonic period 30 min.The chromosome number of European blackberry is 28,tetraploid.
【Fund】: 中波政府间科技合作项目(AM30-033)
【CateGory Index】: S663.2
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