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《Journal of Agricultural University of Hebei》 2008-01
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Induction of polyploid in Ziziphus jujuba Mill. by treating callus

NING Qiang1,2,LIU Meng-jun1,LIU Xiao-guang1,ZHAO Jin1 (1.College of Horticulture,Agricultural University of Hebei,Baoding 071001,China;2.Vocation College of Political Science and Law of Hebei,Shijiazhuang 050061,China)  
By treating Z.jujuba cv.Dongzao and Z.jujuba cv.Lizao with solution of 4.0 mg/L TDZ+2.0 mg/L AgNO3,the callus was obtained.The optimum concentration and time of colchicine treatment for Lizao was: 0.05% and 24 h,percentage of variation was 13.3%.The optimum concentration and time of colchicine treatment for Dongzao was: 0.05% and 24 h, the percentage of variation was 6.7%.Compared with their original diploid plants,the induced tetraploid plants showed that the leaves became longer,wider and thicker.And the serration of the leave became larger than diploid,but the serration number per centimeter was less than normal leaves.The leave index showed smaller than diploid.From the cytological observation,the chromosomes of the Lizao and Dongzao were all 24,but the variation plant became 48.The stomata density obviously decreased,and the guard cells were larger and the chloroplasts in the guard cells were more than the diploid.
【Fund】: 国家科技攻关项目(2001BA502B09-04);; 河北省科技攻关项目;; 河北农业大学科技将帅计划;; 河北农业大学科学发展基金
【CateGory Index】: S665.1
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