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《Journal of Agricultural University of Hebei》 2008-04
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Molecular cloning,prokaryotic expression,and enzyme activity assay of fps from Artemisia annua strain 001

HAN Jun-li~(1,2) LI Zhen-qiu~(2,3) YE He-chun~2 LI Guo-feng~2 (1.Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China;2. Key Laboratory of Photosynthesis and Environmental Molecular Physiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China; 3.College of Life Sciences,Hebei University,Baoding 071002,China)  
Farnesyl diphosphate synthase(FPS)is a key enzyme in the biosynthetic pathway of arte- misinin,a new effective antimalarial drug.In this work,the fps was cloned from Artemisia annua strain 001 by RT-PCR; prokaryotic expression of FPS,purification,and enzyme activity assay were carried out.The results indicated that the homology of amino acid deduced from this FPS gene was 98.83% and 99.42% respectively to other two reported Artemisia annua fps; the His-tag FPS was expressed with high level,high solubility,and FPS enzyme activity.
【Fund】: 天津商业大学引进人才科研启动项目资助
【CateGory Index】: S567.239
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