Screening of antagonistic strains and inhibition of combination fermentation against Phytophthora infestans
GUO Hui-jing~1 JIANG Ji-zhi~1 LI Xiang-bin~1 LI Hong~2 (1.College of Life Sciences,Hebei University,Baoding 071002,China; 2.Shanghai Zoo,Shanghai 200336,China)
Different antagonistic microorganism strains were selected according to their inhibition ef- fect on growth of Phytophthora infestans(Mont.)de Bary by using dual-culture method.Among 36 species,16 species showed the evident inhibition effect.The inhibition rates of B3282,Bacillus sp.,and A32810 were more than 70%,and rates of A4228,A32912,A5253,and A32914 were more than 60%.However,there were only fermentations from 3 of 36 species,A.brassicae,A. kikuchiana,and A32914 displaying remarkable suppression.And their inhibition rates were 80.51%,70.20%,and 48.91%,respectively.It was found that some of combination fermenta- tions from two species microorganism's fermentations showed significantly synergistic or cooperation inhibition to P.infestans.The treatment of A.brassicae fermentation in combination with A. kikuchiana fermentation had the strongest inhibition,and the rate of which reached up to 90.38 %.