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《Journal of Agricultural University of Hebei》 2009-06
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Study of oxidative stress on injury in intestinal epithelial cell and antioxidation in vitro

CHEN Qun,YU Wei,YU Xiu-fang,LIU Hai-yan(Institute of Animal nutrition,Jilin Academy of Agricultural Science,Gongzhuling 136100,China)  
The objective of this study was to investigate the direct oxidative injury of free radicals on rat intestinal epithelial cells(IEC),and antioxidation by glutathione(GSH)in vitro.The results show that X/XO supplementation significantly inhibited the proliferation of IEC and activity of catalase(CAT),but increased the level of Ca2+ compared with the control group(P0.05).There was a slight increase in the activity of super oxide dismutase(SOD) by the low concentration of XO(10 U/L) alone-treated group(P0.05),while supplementation of a high concentration of XO(40,70 U/L) significantly decreased the activity of SOD compared with XO(10 U/L) and the control group(P0.05).Presence of GSH failed to affect the activity of SOD corresponding X/XO(10 U/L) alone-treated.The increase in cell proliferation was also observed in IEC.These results strongly suggest that oxidative stress could induce injury in IEC.Moderate oxidative stress might have selectively stimulated the synthesis of antioxidant enzyme,but significantly decreased cell proliferation.Additions of GSH showed significant protection against oxidative stress in vitro.
【CateGory Index】: R363
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Liao Xianping,She Yaxiong and Li Ming.Department of Urology,Guangdong Provincial Corps Hospital,Chinese People's Armed Police Forces,Guangzhou 510507,China;Comparison of several drugs in maintaining calcium homeostasis in hypoxic cells[J];MEDICAL JOURNAL OF THE CHINESE PEOPLE'S ARMED POLICE FORCES;1999-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WEI Dong et al (College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin 130062);Production of Polyclonal Antibody of NFLX[J];Journal of Anhui Agricultural Sciences;2007-04
2 ZHAN Ai-jun et al(Technical Center of Animal and Plant Quarantine Inspection,Shenzhen CIQ,Shenzhen,Guangdong 518010);Preparation of Monoclonal Antibodies against HA of H9N2 AIV and Identification of its Subtypes[J];Journal of Anhui Agricultural Sciences;2008-03
3 WEI Dong et al(Hebei North University,Zhangjiakou,Hebei 075000);Preparation of Polyclonal Antibody against Amoxicillin[J];Journal of Anhui Agricultural Sciences;2008-09
4 HU Xiao-miao et al (Animal Science and Technology College of Anhui University,Hefei,Anhui 230036);Purification and Antibody Preparation of Canine Serum IgG and its Identification[J];Journal of Anhui Agricultural Sciences;2008-21
5 Liu Yin-Xing1, Fan Dong-Mei1, Xiong Dong-Sheng1, Xu Yuan-Fu1, S hao Xiao-Feng1, Xu Yuan-Sheng1, Peng Hui1, Yang Ming1, Qin Lan2, Zhu Zhen-Ping1, Yang Chun- Zheng1 1.The National Laboratory of Experimental Hematology,Institute of Hematology,Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin,300020, P.R.China 2.Department of Nuclear Medicine, The First Hospital of Tianjin, Tianjin,300192, P.R.China;Apoptosis of Human B Cell Lymphoma Raji Cells Induced by Monovalent Anti-CD20 Antibody[J];Chinese Journal of Cancer;2003-12
6 LI Hua , YU Jian-kun , HONG Chao , et al. (Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118,China);Development and Primary Application of ELISA Method for Detecting Residual Calf Serum Protein[J];Labeled Immunoassays and Clinical Medicine;2005-02
7 LI You tian 1* , XU Dan 1, ZHAO Li 1,LI Hong lin 1, DONG Yu xiang 1, HONG Ying jie 1 ZHANG Er li 1, ZHANG Dian jun 1,XU Cai yun 2, GAO Wen yan 1, HUO Yu ping 1, LI Qiu bo 1,LI Xiao chun 1 (1. Department of Chinese Traditional Medicine,First Hospital, Jilin University, Changchun 130021,China;2. Department of Animal Laboratory, School of Basic Medical Sciences,Jilin University,Changchun 130021,China);Effects of Sanshen Ziwei Yin on G cells and D cells of gastric mucosa in rats with chronic atrophic gastritis[J];Journal of Jilin University of (Medicine Edition);2004-01
8 GAN Lu *,CHEN Xing,WANG Zhiling,et al. *Bethune Military Medical College,Shijiazhuang 050081,China;The Preparation and Evaluation of Monoclonal Antibody against Calmodulin[J];Journal of Bethune Military Medical College;2003-01
9 XU Yong JI Biansheng SHI Leming et al Department of Pharmacology,Binzhou Medical College,Binzhou 256603;Protective effects of imipramine on glutamate-induced neurotoxicity in cerebral cortical neuronal cultures[J];Journal of Binzhou Medical College;2005-02
10 WANG Guoxiang XU Yong LI Shucui et alDepartment of Pathophysiology,Binzhou Medical College,Binzhou 256603;Protective effects of amitriptyline on the injury of hypoxia/hypoglycemia in cultured rat cerebral neurons[J];Journal of Binzhou Medical College;2006-04
China Proceedings of conference Full-text Database 3 Hits
1 Qiu Yan~1 Dong Fa-ming~1 Yang Zi-jun~1 Liu Feng-jun~1 Hu Yuan-liang~2 Cui Bao-an~3 (1.College of Animal technology,Henan Science and Technology University,Luoyang,471003,China; 2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,210095,China; 3.College of Animal Husbandry and Veterinary Medicine,Henan Agricultural University,Zhengzhou,450002,China);Effects of Four Polysaccharides on Proliferation and Production of Nitric Oxide of Splenic Lymphocytes in Chicken[A];[C];2008
2 Ye Liu Shoufeng Zhang Fei Zhang Rongliang Hu Laboratory of Epidemiology,Veterinary institute,Academy of Military Medical Sciences,1068 Qinglong Road,Changchun 130062,P.R.China;Development of a direct competitive ELISA kit for monitoring the level of rabies-neutralizing Antibody in Animals[A];[C];2008
3 Peng Fuhu,Wang Zheng,Bi Dingren ((State Key Laboratory of Agricultural Microbiology,China;College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China));Studies on immunochromatographic method for rapid detection of H9 subtye avian influenza viruses[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Liao Xianping; et al(Department of Pediatric Surgery,Xinhua Hospital,Shanghai Second Medical University,Shanghai;Protection of Intestinal Energy Metabolism by ATP-MgCl_2 and Verapamil Against Ischemic Damage[J];ACTA UNIVERSITATIS MEDICINALIS SECONDAE SHANGHAI;1995-02
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