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《Journal of Agricultural University of Hebei》 2010-03
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The genetic transformation of embryonic tip via particle bombardment in cotton

WANG Zhen-yi,WU Li-zhu,WANG Xing-fen,ZHANG Gui-yin,LIU Jian-feng,MA Zhi-ying (North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Key Laboratory of Crop Germplasm Resources of Hebei,Agricultural University of Hebei,Baoding 071001,China)  
In order to establish a new cotton genetic transformation system,GhDAHPS gene from cotton,which was demonstrated to be related with verticillium wilt resistance,was tried to introduce into cotton via particle bombardment using embryonic tip explants.The results showed that 90 out of 309 embryonic tips were obtained after kanamycin selection culture,these resistant plants was further tested by PCR analysis,5 out of 90 transformed plants were PCR positive,PCR positive rate was 1.6%.This study showed that the method of cotton mature seed embryonic tip as transformation receptors was certainly feasible.Compared with other transformation methods,it has some advantages such as simple operation steps,more quickly growth of the transformed seedlings and more efficient transformation cycle.
【Fund】: 河北省科技支撑计划重大项目(06220113D);; 河北省自然科学基金重大项目(C2006001034)
【CateGory Index】: S562
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