Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Agricultural University of Hebei》 2010-03
Add to Favorite Get Latest Update

The genetic transformation of embryonic tip via particle bombardment in cotton

WANG Zhen-yi,WU Li-zhu,WANG Xing-fen,ZHANG Gui-yin,LIU Jian-feng,MA Zhi-ying (North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Key Laboratory of Crop Germplasm Resources of Hebei,Agricultural University of Hebei,Baoding 071001,China)  
In order to establish a new cotton genetic transformation system,GhDAHPS gene from cotton,which was demonstrated to be related with verticillium wilt resistance,was tried to introduce into cotton via particle bombardment using embryonic tip explants.The results showed that 90 out of 309 embryonic tips were obtained after kanamycin selection culture,these resistant plants was further tested by PCR analysis,5 out of 90 transformed plants were PCR positive,PCR positive rate was 1.6%.This study showed that the method of cotton mature seed embryonic tip as transformation receptors was certainly feasible.Compared with other transformation methods,it has some advantages such as simple operation steps,more quickly growth of the transformed seedlings and more efficient transformation cycle.
【Fund】: 河北省科技支撑计划重大项目(06220113D);; 河北省自然科学基金重大项目(C2006001034)
【CateGory Index】: S562
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 YAO Yan et al(College of Life Science,Shihezi University,Shihezi,Xinjiang 832003);Study on the Improvement of Embryonic Tip Regeneration System of Soybean[J];Journal of Anhui Agricultural Sciences;2007-21
2 ZHANG Dong-xu~1 ZHANG Jie~1 SHANG Lei~1 ZHANG Yun-wei~1 YANG Chun-yan~2 WANG Dong-mei~1 (1.College of Life Sciences,Agricultural University of Hebei,Baoding 071001,China;2.Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,China);Study on the regeneration system of soybean embryonic tips[J];Journal of Agricultural University of Hebei;2008-04
3 MA Xiao-hong,YAO Lu-ming,WU Tian-long(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China);High Frequency Plant Regeneration from Whole Cotyledonary Node Explants and Comparison with Cotyledonary Node and Embryonic Tip Regeneration System in Soybean [Glycine max(L.)Merrill][J];Soybean Science;2008-03
4 SUN Wen-li1,2,3,LIU Yu-hui2,3,WU Yuan-hua1,FU Jun-fan1 (1.Plant Pathology Department, Shenyang Agriculture University, Shenyang 110161,China; 2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081,China; 3.Important Scientific Engineerings of Crops Gene Resources and Gene Improvement of China,Beijing 100081,China);A Pilot Study of An Soybeans Embryonic Tip Regeneration System[J];Hubei Agricultural Sciences;2008-06
5 YU Ya1,LIU Chuanliang2,MA Zhiying1,LI Fuguang2*(1. College of Agriculture, AUH,Baoding, Hebei 071001,China;2. Cotton Research Institute, CAAS,Anyang, Henan 455112, China);The Culture of Cotton Apical Meristem and its Application in Particle Bombardment Transformation[J];Acta Gossypii Sinica;2003-05
6 LIU Chuan-liang, YU Ya, WANG Yu-fen, GENG Li-zhao, LI Fu-guang (Chinese Cotton Research Institute, CAAS; Key Laboratory of Cotton Genetic Improvement ,Ministry of Agriculture, Anyang Henan , 455004, China; Agricultural University of Hebei, Crop Genetic Breeding Department, Baoding Hebei 071000,China);Study on Transformation of Meri- stem via Gene-gun Bombardment[J];Acta Gossypii Sinica;2003-06
7 Wu Jingyin ;Zhu Weiming ;She Jianming ;Cai Xiaoning (Instfrute of Agrobiological Geneticsand Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014)Zhu zhen ;Li Xianghui (Institute of Genetics,Academia Sinica,Beijing,100101);Culture of Apical Meristems of Gossypium hirsutum and Its Applica-tion in Gene Imtroduction[J];ACTA GOSSYPII SINICA;1994-02
8 GUO Yu-Long1, 2 LU Xiu-Min1* ZHU Qin-Long2 LI Ming-Yang2 PEI Yan1 (1.Center of Biotechnology, Southwest Agricultural University, Chongqing 400716, China; 2. Horticulture Cellege, Southwest Agricultual University, Chongqing 400716, China);Genetic Transformation of Cotton Embryogenic Callus by Particle Bombardment and Regeneration of Transgenic Plants[J];Journal of Agricultural Biotechnology;2005-02
9 GUO Dian-Jing1; Fu Rong-Zhao1; LI Wen-Bin1 ;CHEN Ying1;ZHANG Xiao-Dong2; ZHANG Li-Ming1; SUN Yong-Ru1(1 Institute of Genetics Chinese Academy of Sciences Bijing 100101)(2Beijing Academy of Agriculture and Forestry beijing 100081);Studies of Regulation of GUS Transient Expression in Wheat andRabbit Defensin(NP-1)Transformation[J];ACTA GENETICA SINICA;1999-02
10 LIU Hai-Kun, WEI Zhi-Ming(National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; Graduate School of the Chinese Academy of Sciences, Shanghai 200032, China);Transgenic Soybean Obtained with Agrobacterium tumefaciens-medi-ated Transformation of Embryonic Tip of Soybean Mature Seeds[J];Acta Photophysiologica Sinica;2004-06
Chinese Journal Full-text Database 10 Hits
1 LI Zhanqi Liu Dajun(Southwest China Normal University,Chongqing 400715,China);Effects of Different Cultural Conditions on culturing of Xiangmian 18 cotton Stem Tip Tissue[J];Anhui Agricultural Science Bulletin;2007-16
2 XUE Yuanchao Liu Dajun(College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China);Initill study on Tip Tissue Cultural Condition of Xiangmian 18 Cotton[J];Anhui Agricultural Science Bulletin;2007-16
3 Bao Man et al.(Department of Life Science,Nanjing Xiaozhuang College,Nanjing 211171,China);The Establishment of Shoot Tip Culture System of Upland Cotton Simian 3[J];Anhui Agricultural Science Bulletin;2009-15
4 ZHAO Fu-yong(College of Life Science,Yangtze University,Jingzhou,Hubei 434025);Establishment of Agrobacterium-mediated Transformation System of Cotton Shoot Tip[J];Journal of Anhui Agricultural Sciences;2009-02
5 ZHAO Fu-yong (College of Life Science,Yangtze University,Jingzhou,Hubei 434025,China);Establishment of High-frequent Regeneration System of Cotton(Gossypium hirsutum L.) Shoot-tip as Explants[J];Journal of Yangtze University(Natural Science Edition)Agricultural Science Volume;2008-04
6 ZHANG Dong-xu~1 ZHANG Jie~1 SHANG Lei~1 ZHANG Yun-wei~1 YANG Chun-yan~2 WANG Dong-mei~1 (1.College of Life Sciences,Agricultural University of Hebei,Baoding 071001,China;2.Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,China);Study on the regeneration system of soybean embryonic tips[J];Journal of Agricultural University of Hebei;2008-04
7 Zhang Yinxia1,2 Song Xiaohua1 Li Yinhui2 Qiu Lijuan2(1.College of Agronomy,Ningxia University,Ningxia750021;2.Institute of Crop Germplasm Resources,CAAS,Beijing10081);ADVANCES ON THE STUDY OF AGROBACTERIU M-MEDIATED TRANSFOR MATION OF SOYBEAN[J];Soybean Science;2006-03
8 SONG Wen-wen,LIU Bao-hui,YANG Ming-liang,ZHANG Ying,WANG Ji-an(Soybean Research Institute,Northeast Agricultural University/Key Laboratory of National Educational Department,Harbin 150030,Heilongjiang,China);Transformation of SPS Gene into Soybean via Agrobacterium-mediated Method[J];Soybean Science;2008-03
9 HOU Wen sheng 1,GUO San dui 2,LU Ming 1(1 Yangling Branch of China Wheat Improvement Center,College of Agronomy,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Biotechnology Research Institute,Chinese Academ;Construction of high-level expression vectors containing sgna gene for use in wheat Transformation[J];Acta Botanica Boreali-occidentalia Sinica;2002-05
10 Huang Yihong1* Liu Chunguang2 Ma Hongxiang1 Liu Genqi2 Zhou Miaoping1 Sun Yongru2 Yu Guihong1 Lu Weizhong11 Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 2100142 Institute of Genetics and Developnental Biology, Chinese Academy of Sciences, Beijing, 100101;Wheat Transformation by Pollen-Tube Pathway and High Effective Screening System[J];Molecular Plant Breeding;2004-06
China Proceedings of conference Full-text Database 1 Hits
1 LIU Chuan-liang, LI Fu-guang, WANG Yu-fen, LI Feng-lian, CUI Jin-jie, LUO Hong-ying (Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture, Cotton Research Institute, CAAS, Anyang, Henan 455112, China);Detection of Bt Toxin and Analysis of Bollworm-resistance of the T_0 Generation of the Plants Harboring Bt Gene[A];[C];2004
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Gui-lan,QIAO Ya-ke,HAN Jin-ling,BI Yan-juan,ZHAO Shu-guang(Hebei Normal University of Science & Technology,Changli 066600,China);Influencing factors on the survival rate of transplanted plants from Agrobacterium-mediated transformation systems of soybean cotyledonary node[J];Journal of Agricultural University of Hebei;2007-02
2 Xu Xiangling Li Xinghua Liu Weihua Li Jilin(Harbin Normal University 150080)Wang Yi(JiLin Agricultural University 130043);STUDY ON TRANSFERRING SOYBEANS MOSAIC VRIOUS COATPROTEIN (SMV-CP) GENE INTO SOYBEANS BY RI-PLASMID[J];Soybean Science;1996-04
3 Yuan Ying 1 Liu Depu 1 Zheng Peihe 1 Xu Wenjing 1 Li Hailong 2 (1. Jilin Province Key Open Labor On Agro-Biotechnology; 2. JiLin Mechanization in Agriculture School, Jilin Province);STUDY ON PLANT REGENERATION FROM SOYBEAN CULTURE[J];Soybean Science;2001-01
4 Zhou Sijun 1 Li Xichen 1 Liu Zhaojun 1 Liu Liyan 1 Yang Qingkai 2 (1 Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 2 Institute of Soybean, Northeast Agricultural University, Harbin 150030);INTRODUCTION OF Bt GENE(cryIA)INTO SOYBEAN BY Agrobacterium-MEDIATED TRANSFORMTION[J];Soybean Science;2001-03
5 Qiu Chengxiang Wu Tianlong (Shanghai Jiaotong University,Shanghai 201101);STUDY ON 6-BA TO THE REGENERATION OF TIP SHOOT OF SOYBEAN[J];Soybean Science;2003-01
6 Wang Ping Wu Ying Wang Gang Ji Jing (Laboratory of Plant Molecule Biology, Quartermaster University, Changchun 130062);CURRENT PROGRESS ON TISSUE CULTURE OF SOYBEAN[J];Soybean Science;2003-02
7 Wu Xiaoxia Li Wenbin * * Zhang Shuzhen (Soybean Research Institute of Northeast Agriculture University,Harbin 150030);THE RESEARCH ADVANCE ON SOYBEAN TRANSGENE IN CHINA[J];Soybean Science;2005-02
8 Luo Zhenfeng Li Qiyun (Northeast Agricultural University, Haerbin 150030;Jilin Academy of Agricultural Sciences, Gongzhuling 136100);COMMERCIALIZATION STATUS AND RESEARCH PROGRESS OF TRANSGENIC SOYBEAN[J];Soybean Science;2005-03
9 Xu Xiangling Gao Jing Liu Weihua Li Jilin (Harbin Normal University 150080);STUDIES ON TRANSFERRING B.t. k-DELTA ENDOTOXIN GENE INTO SOYBEAN WITH Ti-PLASMID PRIMARIL[J];SOYBEAN SCIENCE;1997-01
10 Xu XianglingZou LianpeiLiu WeihuaLi Jilin (Harbin Normal University 150080);A PRELIMINARY STUDY ON TRANSFERRING CHITINASE GENE INTO SOYBEANS[J];SOYBEAN SCIENCE;1999-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved