Establishment of stretch injury model of in vitro cultured primary liver cells and observation on changes of their cytoskeleton and viscoelasticity
MA Xiao lin, YANG Zhi huan, WANG Zheng guo,et al. (Department of General Surgery, Daping Hospital, Third Military Medical University, Chongqing,China 400042)
Objective To establish the stretch injury model of cultured liver cells,observe the injury of liver cells and changes in their cytoskeleton and viscoelasticity,and explore the mechanisms.Methods After the primary liver cells were cultured in BioFlex culturing plate, they were impacted with a small biological impacting machine with pressures of 150 kPa and 250 kPa respectively. The morphological observation on changes of arrangement and distribution of F-actin microfilament of the cytoskeleton in normal liver cells and those with stretch injury was conducted by using a laser con-focal microscope. Meanwhile, the viscoelasticity of the liver cells was determined.Results After the stretch injury, most of the liver cell microfilaments were broken. Furthermore, their network structure disappeared. The cells shrank to be shaped like flakes or there were only some big microfilament branches left. The viscoelasticity of the liver cells was significantly decreased.Conclusion The remarkable decrease of the viscoelasticity and breaking of cytoskeleton in the liver cells might be the molecular biological mechanism of changes of dynamic features in the liver cells after stretch injury.
【CateGory Index】： R-332