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《Journal of Traumatic Surgery》 2008-01
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Experimental study on the changes of IκK-β/NF-κB in alveolar macrophagesin the process of ischemia-reperfusion injury following hemorrhagic shock

YIN Wen,TONG Wei,XIONG Li-ze,et al.(Department of Emergency,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China)  
Objective To study the molecular changes of IκK-β/NF-κB pathway in alveolar macrophages(AMs) in the process of I/R and explore the possible mechanism of inflammatory lung injury.Methods The rabbit model of I/R was established,AMs were separated and cultured.Hybridization was applied to explore IκK-βmRNA expression in AMs;EMSA was used to detect NF-κB translocation and ELISA was used to detect levels of TNF-α and IL-6 in the supernatant of cell cultures.Finally,inflammatory injury in the lung was studied under microscope.Results The index of IκK-β,NF-κB,TNF-α,IL-6 in the model group increased significantly comparing with that in the control group.More severe inflammatory changes presented in the lung tissues of models of I/R than that in the control group.Conclusion AMs are the major sensitive cells in the changes of NF-κB signal pathway and plays critical role in the inflammatory injury.IkappaB kinase(IκK) activation contributes to NF-κB translocation,thus leads to the release of multiple cytokines and finally causes inflammatory injury in the lung tissue.
【Fund】: 军队“十一五”医药卫生科研基金项目(06AM226);; 军队“科技攻关”医药卫生科研基金项目(06G086)
【CateGory Index】: R641
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