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Studies on the application of orthogonal design in additive experiment of Medicago sativa silage

ZHANG Xin-ping~(1),LI Xiang-lin~(2),WAN Li-qiang~(2)(1.Pratacultural College of Gansu Agricultural University,Lanzhou 730070,China;2.Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100094,China)  
Orthogonal test of 4 factors and 4 levels was included in this experiment,they were wilting time(water content was 80.3%,71.2%,62.5%and 40.3%respectively),adding dose of lactic acid bacteria(0 cfu/g,10~(6) cfu/g,10~(7)cfu/g,10~(8) cfu/g),cellulose(0 g/kg,0.025 g/kg,0.05 g/kg,0.1 g/kg)and glucose(0 g/kg,10(g/kg),5 g/kg,20 g/kg)respectively.The results demonstrated that among four water combinations,ensiling quality of wilted Medicago sativa(water content was 71.2%) was the best,because it reduced the content of NH_(3)-N in silage and produced much lactic acid bacteria.The effect degree of 4 factors on Medicago sativasilage from big to small was glucose,water content,cellulose and lactic acid bacteria respectively.In orthogonal test,the best combination was gained when water content of Medicago sativa,adding dose of lactic acid bacteria,cellulose and glucose was 71.2%,10~(6) cfu/g,0.1 g/kg and 20 g/kg respectively.
【Fund】: 科技部奶业重大专项子课题“苜蓿和饲用玉米青贮新技术研究与产业示范”(2002BA518A03-06)
【CateGory Index】: S816.7
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