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《Grassland and Turf》 2007-02
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Determining organic acid in alfalfa silage by HPLC

XU Qing-fang1,2,YU Zhu1,HAN Jian-guo1,BAI Chun-sheng1,XUE Yan-lin1,XUN Gui-rong3 (1.Institute of Grassland Science,China Agricultural University,Beijing 100094,China;2.College of Animal Science and Technology of Shanxi Agricultural University,Taigu,Shanxi 030801,China;3.Forage and Fodder Station of Tianjin,Tianjin 300210,China)  
Lactic acid,acetic acid,propionic acid,and butyric acid content in alfalfa silage juices were determined simultaneously by using HPLC(Shodex KC-811 column,3 mM perchloric acid as mobile phase,1 mL/min flow rate,L sampling size,column temperature at 50 ℃,Shimadzu SPD-10A detector,and wave length at 210 nm).The results showed that the organic acids were separated well and the sensitivity and recovery were high;the value were reliable;and the operation were easy.
【Fund】: 国家“948”项目“退化草地综合改良与建设配套技术”(202099);; 北京市教委共建项目(XK100190552JD100190531);; 国家科技成果转化项目“苜蓿发酵液裹包青贮技术的转化”(2002710020908);; 天津市重大农业科技合作项目“紫花苜蓿拉伸膜青贮技术推广应用”(0202020208)
【CateGory Index】: O657.72
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Chinese Journal Full-text Database 2 Hits
1 Wang Hongli, Li Chengyun;A determination method based on gas chromatography for analysis of organic acids in alfalfa silage[J];Feed Industry;2008-16
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