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《Grassland and Turf》 2008-05
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Effects of phytohormones screening on callus induction of alfalfa with orthogonal design

CHAI Yan-wen1,MA Hui-ling1,XIE Xiao-dong2,LIU Yong-cai1(1.College of Pratacultural Science,Gansu Agricultural University,Lanzhou 730070,China;2.College of Life Sciences,Lanzhou University,Lanzhou 730000,China)  
The L9(34) orthogonal design was adopted to enhance the frequency of callus induction from alfalfa(Gannong No.3,Peru and Hetian) and MS was used as the elementary medium,added with different concentrations of 6-BA,NAA and KT,and the hypocotyl as explant.The results showed that:MS+2,4-D(2.0 mg/L)+6-BA(0.5 mg/L)+NAA(1.0 mg/L) was the best medium for Peru and Gannong No.3 and the effect sequence of three phytohormones was 6-BANAAKT.MS+2,4-D(2.0 mg/L)+6-BA(1.0 mg/L)+KT(1.0 mg/L)+NAA(1.0 mg/L) was the best medium for Hetian and the effect sequence of three phytohormones was 6-BAKTNAA.The results provided a basic foundation for producing transgenic alfalfas callus.
【Fund】: 甘肃省农牧厅农业生物技术研究与应用开发项目-紫花苜蓿抗病转基因植株的选育(GNSW-2007-03)资助
【CateGory Index】: S541.9
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