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《Grassland and Turf》 2016-02
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Effects of combined application of phosphate and microbial fertilizer on alfalfa production in cold and arid regions in Qinghai-Tibet Plateau

CHEN Juan-li;SHI Shang-li;QI Juan;College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Centers for Grazingland Ecosystem Sustainability;  
Taked alfalfa(Gold Queen)as sample,the paper studied the effect of nitrogen fertilizer,phosphate fertilizer,mixed bacterial fertilizer and mixed phosphate with bacterial fertilizer on the growth and quality of alfalfa and soil microbial biomass in cold and arid regions in Qinghai-Tibet Plateau compared with the treatment without fertilizer.The results showed that soil microbial biomass,height,fresh/dry weight and crude protein content of the alfalfa were all increased after various fertilizer treatments,while the content of NDF and ADF were all decreased.The soil microbial biomass in 0~10cm soil layer were higher than that in 10~20cm soil layer.After applyed 25% phosphate and mixed bacterial fertilizer,the crude protein content could reach 20.58%,which was higher about 3.72% than the contrast,the content of NDF decreased to 1.69%~4.71%.Applied mixture of phosphate and mixed bacterial fertilizer,the content of NDF was lower than single phosphate and higher than single mixed bacterial fertilizer,the content of ADF decreased to 0.05%-9.98%,the content of ADF with mixture phosphate and bacterial fertilizer was significantly lower than that of single phosphate or mixed bacterial fertilizer.After applyed 75% phosphate and mixed bacterial fertilizer,the content of ADF was the lowest.The soil microbial biomass after applied mixed phosphate fertilizer and bacterial fertilizer was significantly higher than that of single phosphate or mixed bacterial fertilizer.Based on gray relational analysis of alfalfa production and soil microbial biomass,the rank of fertilizer treatments was 50% phosphate fertilizer+mixed bacterial fertilizer phosphate fertilizer25% phosphate fertilizer+mixed bacterial fertilizernitrogen fertilizer75% phosphate fertilizer+ mixed bacterial fertilizer mixed bacterial fertilizerCK.
【Fund】: 公益性行业(农业)科研专项(201003023);; 现代农业产业技术体系建设专项资金(CARS-35)资助
【CateGory Index】: S541.9
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