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《China Herbivores》 2007-02
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Phylogenetic Relationship and Genetic Diversity of Chinese four domestic donkeys using mtDNA D-Loop

Sun Wei-li,Yang Bo-hui,Liang Chun-nian,et al(Institute of Lanzhou Animal Science and Veterinary Pharmaceutics Chinese Academy of Agricultural Sciences,730050)  
To study the origin and genetic diversity of Chinese domestic donkeys using mtDNA D-loop.In order to provide scientific materials for the protection and utilizing of Chinese domestic donkey breeds resource.We sequenced the mitochondrial D-loop for 95 donkeys from four breeds.The alignment was carried out by clustalX.Phylogenetic analysis,haplotypes and nucleotide diversity were accomplished by Dnasp 4.10.MEGA3.1 was used for constructing N-J tree.According to the Genbank reference sequence(X97337),there are 33 polymorphic sites in 95 sequences of 385bp long,including 31 locations of transitions,one transversion and one insertion.31 haplotypes were discovered in 95 sequences.The proportion is 32.6%.The highest haplotype proportion is 100% in DZ breed and the lowest is 42.42% in LZ breed.The analysis of N-J tree shows that two obvious branches are taken on in Chinese domestic donkeys.Therefore Chinese domestic donkeys may have two maternal ancestral populations.It is demonstrated that Chinese domestic donkey breed has closer relationship with African donkeys than Asia donkeys,and the Somali wild donkey may be the ancestor of Chinese domestic donkeys.
【Fund】: 科技部国家基础工作专项“西部稀缺野生偶奇蹄动物遗传资源数据库建设”
【CateGory Index】: S822
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