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Cloning of Leptin cDNA from the adipose of Huanghuai goat

Chen Qi-xin1,Shi Xiao-wei2,Zhao Qiao-hui2,et al(Henan Agricultural University,Zhengzhou 450002;Gansu Agricultural University,Lanzhou 730070)  
According to the genomic sequence of Leptin of goats which published on GenBank,We designed and synthesized a pair of primers.The cDNA encoding the mature protein of Leptin was amplified from the total RNA abstracted from adipose tissue of Huanghuai goat by RT-PCR technique and cloned into pMD18-T vector.The positive recombinant clones were characterized by PCR,double enzyme digestion and sequencing.The sequence analysis showed that the coding cDNA of goat Leptin mature protein consisted of 441 nucleotides and encoded 146 amino acids.Corresponding sequence comparisons of goat with mouse,human,pig,cattle and sheep demonstrated that the homologies of the nucleotides were 82.22 %,87.76%,92.97%,96.15%,and 98.64% respectively,while the homologies for the encoded amino acids were 84.25%,86.99%,92.47%,97.95% and 100.00% respectively.It can therefore be concluded that goat Leptin is a highly conservative gene in the evolutionary history.The successive cloning of goat mature protein coding cDNA from the adipose tissue lay a foundation for analyses of its full structure,gene expression and control.
【Fund】: 河南省教育厅自然基金(2007230003);; 河南省科技攻关项目(0524030011)资助
【CateGory Index】: S827
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