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《China Herbivores》 2009-04
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AFLP Analysis on the Genetic Polymorphism of Kazak,Aletai & Bashibai Sheep in Xinjiang

Fu Yong1,Xia Re-fu2,Xiao Fei1,et al(1.Institute of Animal Science and Technology,Shihezi University,Xinjiang 832003;2.Aletai Animal and Veterinary Station)  
A total of 11 AFLP primer combinations were used to detect genetic variation of pooled DNA in a sample of 3 indigenous sheep breeds DNA pool of genetic variation,and AFLP DNA fingerprinting of each sheep breed was constructed.Polymorphic bands and genetic similarity coefficient of 3 sheep breeds were detected and calculated from AFLP data,and UPGMA cluster analysis was also performed.The 11 primer combinations generated 310 polymorphic bands,28.18 polymorphic markers were detected by one primer combination on the average,in the range of 25 to 34,including 32 polymorphic bands,accounting for amplification of the total number of 10.32 percent with each of the primers amplified polymorphic with an average of 2.909,the highest genetic similarity coefficient of 0.699 existed between Kazak sheep and Aletai sheep,while the lowest similarity coefficient of 0.592 was revealed between Kazak sheep and Bashibai sheep,the results were in consonance with breeding history,differentiation and geographical distribution of sheeps.
【Fund】: 兵团基础研究项目(2007JC02);; 石河子大学动植物育种专项(xjs2007-yz02)资助
【CateGory Index】: S826.2
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