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《Chinese Journal of Materials Research》 2012-02
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Compare of Biocompatibility of the Different Dental Alloys

HONG Yansong~1 LI Xiumei~1 MENG He~2 ZHAN Desong~(1*) AI Hongjun~1 1.School of Stomatology.China Medical University.Shenyang 110002 2.School of Stomatology.Hebei United University.Tangshan 063000  
Cultivated L929 cells were fostered by Ag-Pd alloys leaching liquor,Ni-Cr alloys leaching liquor,Co-Cr alloys leaching liquor,self-made 2~# alloys leaching liquor(each group sample capacity is 8).Cultivated L929 cells by RPMI 1640 cell medium containing 10%fetal calf serum were taken as the negative control.The cell proliferation rates were detected by MTT,the activity of caspase-3 after 4 h,7 h,24 h,48 h was calculated.The cells condition after 48h was observed by fluorescence microscope.To analyze the relationship between dental alloys biocompatibility and apoptosis correlation factor caspase-3 it was assisted by time gradient.The results show that cell quantity of each group are difference,there are lots of apoptosis cells in each group,but obviously different,orange dead cells can be seen in Ni-Cr alloys group after 48h.Cytotoxicity of each group is grade 0,the order of cell proliferation rates of groups is:Co-Cr alloys group2~# alloys groupAg-Pd alloys groupNi-Cr alloys group.The activity of caspase-3 in each group is obviously different(P 0.01).The order from high to low is:Co-Cr alloys group2~# alloys groupAg-Pd alloys groupNi-Cr alloys group.The activity of caspase-3 on the spot of 4 hour,7 hour,24 hour and 48 hour is obviously different(P 0.01).The order from high to low was:4 hour,48 hour,7 hour,24 hour.The order of these dental alloys biocompatibility from high to low is:Co-Cr alloys group2~# alloys groupAg-Pd alloys groupNi-Cr alloys group.Biocompatibility is mainly affected by Ni and Ag ions.Detecting dental alloys biocompatibility by caspase-3 assisted by time gradient,the result is the same as that by MTT.
【CateGory Index】: TG131
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