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《Acta Sericologica Sinica》 1990-01
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STUDIES ON BLOOD ESTERASE ISOZYME OF BOMBYX MORI

Yang Rui-li Xu Jing-fei (Department of Sericulture, Anhui Agricultural College)  
In this experiment, by means of the discontinuous system of polyacrylamidc gel clcctrophorcsis, we analysed the blood cstcrase zymograms with 24 strains or hybrids of the silkworm, Bombyx mori L. from the third instar larva to the pupa. The results showed that the band types of cstcrase isozyme arc complicated in the blood of the silkworm. We observed 22 isozyme bands which can be divided into 5 regions: A, B, C, D and E, according to the stain features as well as the moving velocity towards the anode. The major thirteen bands can be dctected during various development stagcs. The activity of major bands decreased, but the other bands (A-region) were clearly present in late pupa. During the period of larva, the numbers and activities of isozyme bands increased with the increase of quantity of mulberry leaves which silkworm took in. And the display of A-2-b band and B-2 band was remarkable. It was guessed that the A-2-b band may be basic band of blood cstcrase isozyme of silkworm, and the activity of A-2-b band was dramatically postivc correlation with the most quantitivc characters.
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