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《Acta Sericologica Sinica》 1991-03
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STUDIES ON THE BACTERICIDAL EFFECT OF ANTIBACTERIAL PEPTIDE D FROM CHINESE OAK SILKWORM,ANTHERAEA PERNYI

Li Wen-chu Lu Keng-ming Huang Zi-ran (South China Agricultural University,Guangzhou 510642 ,China) Dai Zhu-ying (Nanjing Normal University, Nanjing 21 0024 ,China) Huang Da-nian (China Natumal Rice Research Institute, Hangzhou 3 1 0003,China)  
Antibacterial peptide D from the haemolymph of Chinese oak silkworm, Antheraea pernyi had a strong bactericidal effect on the bacterium of rice leaf blight disease,Xanthomonas campestris Pv oryze and potato wilt disease, Pseudomonas solanacearum. The bactericidal effect was related to the amount of antibacterial peptide and environment such as temperature,pH and nutrition. Ultra-thin section technique was used to study the mechanism of bactericidal effect of antibacterial peptide, the primary targets were the bacteria] outer layer and cell membrane,especially sensitive to the both ends. At initial action stage, the receptor sites were seriously injured and some microtubules were observed. Potassium ion of inner cell leaked out, intracellular subatances were pressed out of the cell subsequently, and cell become empty vesicles.
【Fund】: 国家自然科学基金
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