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《Acta Sericologica Sinica》 2002-04
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RAPD Analysis of DNA Polymorphism of Antheraea pernyi

Liu Yanqun 1,2 Lu Cheng 1 Xiang Zhonghuai 1( 1The Key Sericultural Laboratory of Agricultural Ministry,Southwest Agricultural University, Chongqing 400716; 2College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110161)  
DNA Polymorphism in each 4 individuals of 4 Antheraea pernyi varieties (He41,Siqing,Qinghuang No.1,Xinghuang) and 3 Bombyx mori varieties (Dazao,C108,7532) was analyzed with Random Amplified Polymorphic DNA(RAPD) technique.The results showed that Antheraea pernyi has abundant DNA polymorphism.The DNA polymorphism of different individuals among different varieties is 80.7%,and that of different individuals within same variety reaches 45.8%~49.4%.The genetic distance (D) value of different individuals within same variety is 0.133~0.238,higher than Bombyx mori which is 0.008~0.081;The D value between different varieties is 0.215~0.382,same as Bombyx mori which is 0.206~0.356. 60% DNA polymorphism of Antheraea pernyi is derived from different individuals within same variety,and 40% from different varieties.
【Fund】: 国家自然科学基金资助项目 ( 30 170719)
【CateGory Index】: S885
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