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《Acta Sericologica Sinica》 2003-04
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cDNA Libraries Construction and Large-scale ESTs Sequencing of the Silkworm

CHENG Daojun XIA Qingyou ZHOU Zeyang LU Cheng XIANG Zhonghuai(Key Sericultural Laboratory of the Agricultural Ministry, Sericulture and Biotechnology College,Southwest Agricultural University, Chongqing 400716)  
The technology of expressed sequence tags (ESTs) was broadly applied in analysis of gene function and expression pattern. Based on the construction technology of non-normalized cDNA library, the cDNA libraries of 13 tissues from silkworm were constructed, and 84 971 ESTs were generated by the large-scale cDNA sequencing. In addition, 27 693 non-repetitive sequences were formed including 10 433 contigs and 17 260 singletons from these ESTs after initial assembly. The large-scale ESTs sequencing provided a valuable resource for the functional genome research and the whole genome sequencing project of silkworm.
【Fund】: 973前期项目 (编号 410 85 8);; 国家自然科学基金 (编号3 0 2 70 691);; 重庆市科委院士基金
【CateGory Index】: S881.2
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