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《Acta Sericologica Sinica》 2003-04
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The Relation Between the Protein Composition of Posterior Silkglands in the Fifth Instar Larva of Silkworm and Cocoon Shell Weight

YAN Xinpei 1 ZHONG Boxiong 1 XU Mengkui 1 GUO Dingguo 2 YAO Guohua 1( 1Laboratory of Molecular Biology of Silkworm and Honeybee, College of Animal Science, Zhejiang University, Hangzhou 310029; 2Silkworm Breeding Reproduction Institute of Guangdong Province, Guangzhou 510640)  
The fifth instar of silkworm is the stage when the cells of posterior silkgland greatly synthesize and secrete fibroin protein, the main component of silk. The protein composition of posterior silkgland in this stage is studied so that the related functional proteins that occur during the secretion of fibroin protein by posterior silkgland will be found and the high production mechanism of cocoon will be elucidated. It was found that protein composition of posterior silkglands from different individuals of the same race and different parts of posterior silkglands from the same individual were almost the same by using two-dimensional electrophoresis of protein and image analysis system. The results suggested that there was little difference among metabolic level of individuals in the same race.Therefore, the difference of cocoon shell weight among individuals in the same race was probably induced by difference of development and growth among individuals, cell number of posterior silkgland and so on. Howerer, the relation between cocoon shell weight among individuals in the same race and metabolism of posterior silkgland in different individuals in the same race was no significantly difference. The protein samples extracted from any individual or any part of posterior silkgland in the same race had no influence on the proteome results by two-dimensional electrophoresis of protein and stain techniques at present.
【Fund】: 国家自然科学基金 (编号 3 0 2 710 0 4)
【CateGory Index】: S881.2
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3 Xia Qingyou;Zhou Zeyang;Lu Cheng; Feng Llchun; Xiang Zhongh ai(The SericuItural Key Laboratory of Ministry of of griculture,College of Sericulture & Silk,Southwest Agricultural University,Chongqing 6307l6,China);A STUDY ON CONDITIONS,AND REPEATABILITY AND INHERITANCE PATTERN OF RANDOM AMPLIFICATION POLYMORPHIC DNA(RAPD)IN F1 OF SILKWORM(BOMBYX MORI.)[J];ACTA SERICOLOGICA SINICA;1996-01
4 GAO Lan 1,FU Jian xi 1,WANG Jian hua 2,WANG Rong 2(1 College of Life Science,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Horticulture Institute of Xian,Lintong,Shaanxi 710603,China);RAPD analysis of tomato F_1 hybrid seeds[J];Acta Botanica Boreali-occidentalia Sinica;2001-03
5 Zhou Qunchu 1 Ma Yanqing 1 Zhang Zhuqing 1 Li Xuefeng 1 Ai Xin 2 (1 Hunan Vegetable Research Institute,Changsha,410125) (2 College of Plant Science and Technology,HNAU,Changsha,410128);Purity Determination of Pepper Hybrid by Ran domly Amplified Polymorphic Technique[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;1999-02
6 DING Qin Xue 1, JIA Yu Feng 1, ZHAO Cong Jian 1,QUE Hai Ping 1, GUO Yao Jun 2, LIU Shao Jun 1 (1. Institute of Basic Medicine, Academy of Military Medical Sciences, Beijing 100850, China; 2.Institute of Biophysics, Chinese Academy of Sci;Comparison of the two dimensional electrophoresis maps of rat spinal cord protein extracted by two different solution systems[J];Bulletin of The Academy of Military Medical Sciences;2001-01
7 Wan Jinghong, Qian Xiaohong, Guo Yaojun, Xing Guichun, Hu Zhiyuan, Qiu Zongyin, He Fuchu Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850;Preliminary establishment and optimization of two dimensional gel electrophoresis(2 DE) for the proteome analysis[J];BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES;1998-04
8 Ouyang Xinxing; Xu Yong;Zhang Haiying Kang; Guobin; Wang Yongjian (National Engineering Research Center for Vegetable, Beijing 100089 );Rapid Identification of Hybrid Purity in Watermelon Using Random Amplified Polymorphic DNA (RAPD)[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1999-01
9 LAN Yan 1,2,3) ,QIAN Xiao Hong 3) ,WANG Ge 1) ,LI Yong 1) , LUO Ling 1) ,LIU Zheng Liang 2) ,HE Fu Chu 1) ( 1) Beijing Institute of Radiation Medicine, Beijing 100850,China; 2) Department of Pharmarcuetic;The Establishment of Sequential Extraction Method in the Proteome Analysis[J];Progress In Biochemistry and Biophysics;2001-03
10 HE Ning Jia, LU Cheng, ZHOU Ze Yang, XIANG Zhong Huai(The Key Sericultural Laboratory of Agricultural Ministry, College of Sericulture, Southwest Agricultural University,Chongqing 400716,China).;The Polymorphic Analysis of Silkworm Genomic DNA by an Effective Method——SADF.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1998-06
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