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《Acta Sericologica Sinica》 2004-01
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Establishment of 2D-PAGE Protein Patterns of Silkworm Embryo During Incubating Stage

YAN Xinpei 1 ZHONG Boxiong 1 CAO Jiashu 2 XU Mengkui 1 SHEN Feiying 1 YAO Guohua 1( 1Laboratory of Molecular Biology of Silkworm and Honeybee, College of Animal Science, Zhejiang University, Hangzhou 310029; 2Laboratory of Cell and Molecular Biology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029)  
Embryo development is always one of the most important subjects in life sciences. The composing of embryo proteins and rule of gene sequential expression at protein level can be discussed by researching the proteins of every embryo development stage of silkworm. The proteins of every embryo development stage were separated and the protein patterns were established employing continuous developmental embryo during incubating stage of silkworm using two-dimensional electrophoresis. The results indicated that there was little difference in structural gene expression from longest stage embryo to teachea colouring stage embryo of silkworm, however, after head pigmentation stage embryo of silkworm, some structural genes of acidic proteins were activated.
【Fund】: 国家自然科学基金 (编号 3 0 2 710 0 4)
【CateGory Index】: S881.2
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