Cloning of Adenine Nucleotide Translocase Gene From Bombyx mori Based on EST Database
CHEN Dafu 1, 3 NIU Baolong 2 WENG Hongbiao 2 MENG Zhiqi 2 L Shunlin 1 ( 1 College of Animal Science, Zhejiang University, Hangzhou 310029; 2 Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021; 3 College of Bee Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002)
According to the relative conservation of homologous gene, a bioinformatics strategy is applied to clone a novel silkworm (Bombyx mori) adenine nucleotide translocase gene (ant) by blasting search of EST database with homologous gene cDNA of Drosophila melanogaster and identified. The full length silkworm ant cDNA of 1 963 bp encodes adenine nucleotide translocase (ANT) of 300 amino acids (aa), which is very conserved in Apis mellifera, Lucilia cuprina, D. melanogaster, Anopheles gambiae and Manduca sexta. The results revealed that it is a convenient technique for cloning novel gene by searching EST database with homologous gene of model living things such as the fruit fly.