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《Acta Sericologica Sinica》 2004-04
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Construction of Nosema bombycis (N.b) cDNA Library and Analysis of ESTs Sequence From Partial Clones

LIU Jiabin PAN Guoqing CHENG Daojun XIA Qingyou ZHOU Zeyang* XIANG Zhonghuai(Key Laboratory of Sericulture of Agriculture Ministry, School of Sericulture and Biotechnology,Southwest Agricultural University,Chongqing 400716)  
Nosema bombycis cDNA library has been constructed with mature Nosema bombycis as material and λTriplEx2 as vector.The titration of the cDNA library was 1 86×106 pfu/mL,the insert fragment length was over 500 bp.180 clones were selected,sequenced and we got 130 useful sequence.After analyzed by BLASTn and BLASTx arithmetic,seven ESTs have high homology with Encephalitozoon cuniculi in amino acid level.These putative genes perhaps existed in Nosema bombycis,at the same time,we also obtained twenty-seven function-unknown sequence.
【Fund】: 重庆市科委院士基金项目 (编号 0 2 -75 5 1);; 重庆市应用基础项目 (编号 73 3 6)
【CateGory Index】: S884.2
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1 Cai Ming-wen Fang Li-xiu Sima Yang-hu~* (School of Life Sciences,Soochow Univesity,Suzhou Jiangsu 215123);Application Actuality and Prospect of EST Library in the Study of Bombyx Mori[J];Jiangsu Sericulture;2007-03
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1 Gao Yongzhen Huang Kewei(Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018)Chang Zhijie(Department of Biological Science and Biotechnology,Tsinghua University,Beijing 100084);Cloning and Characterization of a Gene Coding Reverse Transcriptase From Nosema bombycis[J];Acta Sericologica Sinica;2002-02
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