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《Acta Sericologica Sinica》 2004-04
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Construction of Nosema bombycis (N.b) cDNA Library and Analysis of ESTs Sequence From Partial Clones

LIU Jiabin PAN Guoqing CHENG Daojun XIA Qingyou ZHOU Zeyang* XIANG Zhonghuai(Key Laboratory of Sericulture of Agriculture Ministry, School of Sericulture and Biotechnology,Southwest Agricultural University,Chongqing 400716)  
Nosema bombycis cDNA library has been constructed with mature Nosema bombycis as material and λTriplEx2 as vector.The titration of the cDNA library was 1 86×106 pfu/mL,the insert fragment length was over 500 bp.180 clones were selected,sequenced and we got 130 useful sequence.After analyzed by BLASTn and BLASTx arithmetic,seven ESTs have high homology with Encephalitozoon cuniculi in amino acid level.These putative genes perhaps existed in Nosema bombycis,at the same time,we also obtained twenty-seven function-unknown sequence.
【Fund】: 重庆市科委院士基金项目 (编号 0 2 -75 5 1);; 重庆市应用基础项目 (编号 73 3 6)
【CateGory Index】: S884.2
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【References】
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1 Cai Ming-wen Fang Li-xiu Sima Yang-hu~* (School of Life Sciences,Soochow Univesity,Suzhou Jiangsu 215123);Application Actuality and Prospect of EST Library in the Study of Bombyx Mori[J];Jiangsu Sericulture;2007-03
【Citations】
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1 Gao Yongzhen Huang Kewei(Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018)Chang Zhijie(Department of Biological Science and Biotechnology,Tsinghua University,Beijing 100084);Cloning and Characterization of a Gene Coding Reverse Transcriptase From Nosema bombycis[J];Acta Sericologica Sinica;2002-02
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【Co-references】
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1 SHEN Wei-feng, NIU Bao-long, WENG Hong-biao, MENG Zhi-qi (Sericultural Research Institute, Zhejiang Academy of Agricultural Science,Hangzhou 310021, China);Cloning 6-phosphogluconate Dehydrogenase(6PGDH)of the Silkworm Bombyx mori by Selecting EST Database[J];Bulletin of Sericulture;2005-01
2 CHEN Dafu 1, 3 NIU Baolong 2 WENG Hongbiao 2 MENG Zhiqi 2 L Shunlin 1 ( 1 College of Animal Science, Zhejiang University, Hangzhou 310029; 2 Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021; 3 College of Bee Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002);Cloning of Adenine Nucleotide Translocase Gene From Bombyx mori Based on EST Database[J];Acta Sericologica Sinica;2004-02
3 YU Dongliang~(1,2) ZHONG Boxiong~(1*) FU Yan~1 WANG Xusheng~3 (~1College of Animal Science,Zhejiang University,Hangzhou 310029,China;~2James D.Watson Institute of Genome Sciences,Zhejiang University,Hangzhou 310029,China;~3College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China);Comparison of ESTs and SNPs From Posterior Silkgland of Silkworm Bombyx mori Between the Strain P50 and C108[J];Acta Sericologica Sinica;2005-04
4 HUANG Zhi-Jun1 DENG Xiao-Juan1 CHEN Fang-Yan1 MU Pei-Qiang1YANG Wan-Yang1 ZHONG Yang-Jin1 XIA Qing-You2 CAO Yang1(1Department of Sericulture Science,College of Animal Science,South China of Agricultural University,Guangzhou 510642,China;2Key Sericultural Laboratory of Agriculture Ministry,College of Sericultureand Biotechnology,Southwest University,Chongqing 400716,China);Analysis on the Protein Expression Profile in Midgut From Silkworm,Bombyx mori, During Larva-pupae Metamorphosis[J];Science of Sericulture;2007-02
5 HUANG Zhi-jun~1,ZHONG Yang-jin~1,DENG Xiao-juan~1,HE Xiang-ren~2,LIANG Hong~1,PAN Zhong~1 (1 College of Animal Science,South China Agric.Univ.,Guangzhou 510642,China;2 Guangdong Agric.Well-Bred Demonstration and Popularize Center,Guangzhou 510520,China);Observation of the Midgut and Silk Gland in Silkworm During Pupal-Adult Metamorphosis[J];Journal of South China Agricultural University;2006-02
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10 Xiaobai Li, Hairui Cui, Minglong Zhang* Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029;Molecular markers derived from EST:their development and applica-tions in comparative genomics[J];Biodiversity Science;2006-06
【Secondary Citations】
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1 WU Ying,SHI Yu liang (Key Laboratory of Neurobiology,Shanghai Institute of Physiology,Chinese Academy of Sciences,Shanghai 200031,China)[;Advances in the study of molecular mechanism of neuroexocytosis[J];Chinese Journal of Neuroscience;2000-03
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